LOCUS       NZ_CP115183            25090 bp    DNA     linear   CON 29-FEB-2024
DEFINITION  Clostridioides difficile R20291 chromosome, complete genome.
ACCESSION   NZ_CP115183
VERSION     NZ_CP115183.1
KEYWORDS    .
SOURCE      Clostridioides difficile R20291 (Clostridium difficile R20291)
  ORGANISM  Clostridioides difficile R20291
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            CP115183.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
            ##gutSMASH-Data-START##
            Version      :: 2.0.0-75c9ef8(changed)
            Run date     :: 2024-12-20 13:41:33
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 1195830
            Orig. end    :: 1220920
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..25090
                     /aStool="rule-based-clusters"
                     /contig_edge="False"
                     /core_location="[1205830:1210920]"
                     /cutoff="2000"
                     /detection_rule="(Complex1_51K and minimum(5, [FMN_bind,
                     FeS, Fer4, Fer4_9, NQR2_RnfD_RnfE, Rnf-Nqr, RnfC_N,
                     SLBB]))"
                     /neighbourhood="10000"
                     /product="Rnf_complex"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      10001..15090
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="(Complex1_51K and minimum(5, [FMN_bind,
                     FeS, Fer4, Fer4_9, NQR2_RnfD_RnfE, Rnf-Nqr, RnfC_N,
                     SLBB]))"
                     /neighbourhood="10000"
                     /product="Rnf_complex"
                     /protocluster_number="1"
     cand_cluster    1..25090
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Complex1_51K and minimum(5, [FMN_bind,
                     FeS, Fer4, Fer4_9, NQR2_RnfD_RnfE, Rnf-Nqr, RnfC_N,
                     SLBB]))"
                     /kind="single"
                     /product="Rnf_complex"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..25090
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="Rnf_complex"
                     /region_number="8"
                     /rules="(Complex1_51K and minimum(5, [FMN_bind, FeS, Fer4,
                     Fer4_9, NQR2_RnfD_RnfE, Rnf-Nqr, RnfC_N, SLBB]))"
                     /tool="gutsmash"
     gene            119..2767
                     /gene="polA"
                     /locus_tag="O9S30_RS05575"
                     /old_locus_tag="O9S30_05575"
     CDS             119..2767
                     /EC_number="2.7.7.7"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_function="GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /GO_process="GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="polA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /locus_tag="O9S30_RS05575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05575"
                     /product="DNA polymerase I"
                     /protein_id="WP_009888908.1"
                     /transl_table=11
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFKM
                     IDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFKINRLE
                     IDGYEADDIIGTVSKKAENNGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEYNFN
                     SVIEKYEMTPAQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENTDKLK
                     GSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMVFGDYDKDELLDKFRYFGFTSLL
                     SRLVDLVDSDDTEHVEEKIEILKLKDTEKFIDEVNKKGQVILKTVRGQGNILEKNIIYI
                     FISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLENMYFDITI
                     AEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDLAEYMGQII
                     CTVKETIPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILDELSVEFKEI
                     IATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNADVLEKLRDKHP
                     IIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRISSTEPNLQNIPI
                     KMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFKHHEDIHTKTASQ
                     VFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKEYIESYFAKYHKIK
                     EFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAMNAPIQGSAADIIKI
                     AMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKEEMENAVNMDVHLDVD
                     LNVGSSWYETK"
     gene            2776..3378
                     /gene="coaE"
                     /locus_tag="O9S30_RS05580"
                     /old_locus_tag="O9S30_05580"
     CDS             2776..3378
                     /EC_number="2.7.1.24"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="coaE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /locus_tag="O9S30_RS05580"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="O9S30_05580"
                     /product="dephospho-CoA kinase"
                     /protein_id="WP_009892906.1"
                     /transl_table=11
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNISIVDADIISRKIFEDKLLL
                     EKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNSLTHPRIREKIISEIEELRKK
                     GENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSKQEALSRINSQMSQ
                     EEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            3371..3925
                     /locus_tag="O9S30_RS05585"
                     /old_locus_tag="O9S30_05585"
     CDS             3371..3925
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /locus_tag="O9S30_RS05585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05585"
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_003438043.1"
                     /transl_table=11
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSKE
                     FNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPETN
                     IRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQTSKY
                     VEKVKNNYEHYNKIYGKKGKN"
     gene            3925..5640
                     /locus_tag="O9S30_RS05590"
                     /old_locus_tag="O9S30_05590"
     CDS             3925..5640
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /locus_tag="O9S30_RS05590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05590"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_009888909.1"
                     /transl_table=11
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKYI
                     NLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSIDI
                     KLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFKARY
                     AFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYYEEVP
                     KTMKNIKVGMVPNEEARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDYECVLF
                     NYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMKDLEFNK
                     DKAIECLAKVEYANINSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEEDRIKKEA
                     EKKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTVNQLSDKDM
                     ENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTSEIETKKAYK
                     DVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIKSNESN"
     misc_feature    5682..5697
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: medium, score: 26.64"
                     /tool="gutsmash"
     misc_feature    5682..5698
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: medium, score: 29.76"
                     /tool="gutsmash"
     misc_feature    complement(5683..5698)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 22.72"
                     /tool="gutsmash"
     gene            5713..5925
                     /locus_tag="O9S30_RS05595"
                     /old_locus_tag="O9S30_05595"
     CDS             5713..5925
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /locus_tag="O9S30_RS05595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05595"
                     /product="heavy-metal-associated domain-containing protein"
                     /protein_id="WP_009888910.1"
                     /transl_table=11
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASVD
                     MKDDISIEKLKEVIAELGFELKGIE"
     gene            6126..6725
                     /locus_tag="O9S30_RS05600"
                     /old_locus_tag="O9S30_05600"
     CDS             6126..6725
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /locus_tag="O9S30_RS05600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05600"
                     /product="hypothetical protein"
                     /protein_id="WP_003419035.1"
                     /transl_table=11
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIINN
                     NIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLDIN
                     KRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKTRED
                     MRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            6953..7324
                     /locus_tag="O9S30_RS05605"
                     /old_locus_tag="O9S30_05605"
     CDS             6953..7324
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyoxalase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /locus_tag="O9S30_RS05605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05605"
                     /product="VOC family protein"
                     /protein_id="WP_003419037.1"
                     /sec_met_domain="Glyoxalase (E-value: 9.8e-09, bitscore:
                     33.3, seeds: 82, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILVY
                     LGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENESMG
                     IYFIADPDNYWIEILPKNH"
     gene            7456..8730
                     /locus_tag="O9S30_RS05610"
                     /old_locus_tag="O9S30_05610"
     CDS             7456..8730
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /locus_tag="O9S30_RS05610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05610"
                     /product="C40 family peptidase"
                     /protein_id="WP_009888911.1"
                     /transl_table=11
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSDC
                     DKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSNQNNPTSSGT
                     TISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTGWAS
                     SQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPGTSYS
                     IIGKLNGGDVVELKSKNNGWYKVKLSSGTIGWVSASYISETNEGTKEKPNSSSNQNSQS
                     NSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYVFKHSVG
                     VSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIHCSGTQTN
                     PNKVKVDNLTSSYWSKVLLGARRFV"
     gene            8841..9785
                     /locus_tag="O9S30_RS05615"
                     /old_locus_tag="O9S30_05615"
     CDS             8841..9785
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /locus_tag="O9S30_RS05615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05615"
                     /product="hypothetical protein"
                     /protein_id="WP_003438046.1"
                     /transl_table=11
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDSK
                     ILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNTKE
                     PEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTYSSF
                     DENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFKEESS
                     FWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNVNEKID
                     TASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            10001..11338
                     /gene="rsxC"
                     /locus_tag="O9S30_RS05620"
                     /old_locus_tag="O9S30_05620"
     CDS             10001..11338
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="rsxC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: SLBB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: Complex1_51K"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: RnfC_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_10"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /locus_tag="O9S30_RS05620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05620"
                     /product="electron transport complex subunit RsxC"
                     /protein_id="WP_003428488.1"
                     /sec_met_domain="Fer4_10 (E-value: 9.8e-08, bitscore: 29.8,
                     seeds: 59, tool: rule-based-clusters)"
                     /sec_met_domain="SLBB (E-value: 1.2e-12, bitscore: 45.2,
                     seeds: 67, tool: rule-based-clusters)"
                     /sec_met_domain="Complex1_51K (E-value: 9.4e-52, bitscore:
                     172.7, seeds: 88, tool: rule-based-clusters)"
                     /sec_met_domain="RnfC_N (E-value: 7.9e-40, bitscore: 132.7,
                     seeds: 53, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 0.00018, bitscore: 19.6,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAPA
                     KPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDFKE
                     ELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVILNGA
                     ECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVAKDYS
                     EIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSIKTGMP
                     LVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMMGIAQYT
                     TEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSLKDDYDTA
                     EYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            11361..12338
                     /locus_tag="O9S30_RS05625"
                     /old_locus_tag="O9S30_05625"
     CDS             11361..12338
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: NQR2_RnfD_RnfE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /locus_tag="O9S30_RS05625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05625"
                     /product="RnfABCDGE type electron transport complex subunit
                     D"
                     /protein_id="WP_003428487.1"
                     /sec_met_domain="NQR2_RnfD_RnfE (E-value: 1.2e-116,
                     bitscore: 387.0, seeds: 472, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLNA
                     LSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGGIF
                     AILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTATPL
                     SFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINYVIPV
                     FYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIYAVLAG
                     LITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            12338..12907
                     /locus_tag="O9S30_RS05630"
                     /old_locus_tag="O9S30_05630"
     CDS             12338..12907
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_process="GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: FMN_bind"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /locus_tag="O9S30_RS05630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05630"
                     /product="RnfABCDGE type electron transport complex subunit
                     G"
                     /protein_id="WP_009888912.1"
                     /sec_met_domain="FMN_bind (E-value: 3.6e-18, bitscore:
                     63.7, seeds: 891, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQENNELR
                     KAVLPEAKEFKEMDKSTYKDLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVMVG
                     ISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQILAIS
                     GATITSTAVTTGVNAAIDVFNSALNK"
     gene            12920..13513
                     /locus_tag="O9S30_RS05635"
                     /old_locus_tag="O9S30_05635"
     CDS             12920..13513
                     /GO_process="GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: Rnf-Nqr"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /locus_tag="O9S30_RS05635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05635"
                     /product="electron transport complex subunit E"
                     /protein_id="WP_003419045.1"
                     /sec_met_domain="Rnf-Nqr (E-value: 5.2e-68, bitscore:
                     226.3, seeds: 650, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLSTA
                     AVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIPLI
                     VVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMTLFG
                     ASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            13528..14103
                     /gene="rsxA"
                     /locus_tag="O9S30_RS05640"
                     /old_locus_tag="O9S30_05640"
     CDS             13528..14103
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="rsxA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: Rnf-Nqr"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /locus_tag="O9S30_RS05640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05640"
                     /product="electron transport complex subunit RsxA"
                     /protein_id="WP_003419046.1"
                     /sec_met_domain="Rnf-Nqr (E-value: 4.5e-62, bitscore:
                     206.9, seeds: 650, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAVT
                     FVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALGVY
                     LPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELADIPE
                     AFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            14119..15090
                     /locus_tag="O9S30_RS05645"
                     /old_locus_tag="O9S30_05645"
     CDS             14119..15090
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: FeS"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: Fer4_9"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: Fer4"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_7"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_21"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1018:4Fe-4S dicluster domain (Score: 44.6; E-value:
                     3.9e-14)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /locus_tag="O9S30_RS05645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05645"
                     /product="RnfABCDGE type electron transport complex subunit
                     B"
                     /protein_id="WP_003428486.1"
                     /sec_met_domain="Fer4_7 (E-value: 5.7e-09, bitscore: 34.2,
                     seeds: 208, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_9 (E-value: 3.4e-08, bitscore: 31.3,
                     seeds: 61, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_21 (E-value: 2.4e-12, bitscore: 44.6,
                     seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4 (E-value: 6.4e-06, bitscore: 23.6,
                     seeds: 528, tool: rule-based-clusters)"
                     /sec_met_domain="FeS (E-value: 4.6e-13, bitscore: 46.4,
                     seeds: 364, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 0.00033, bitscore: 18.8,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLPG
                     ANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKVIC
                     KGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDGIAV
                     IDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCGMCVK
                     ACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCVGCTVC
                     KKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     misc_feature    complement(15208..15225)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.94"
                     /tool="gutsmash"
     gene            15310..15894
                     /locus_tag="O9S30_RS05650"
                     /old_locus_tag="O9S30_05650"
     CDS             15310..15894
                     /EC_number="3.6.1.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /locus_tag="O9S30_RS05650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05650"
                     /product="Maf family protein"
                     /protein_id="WP_003428485.1"
                     /transl_table=11
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLVM
                     RLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVITG
                     ISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLVEEI
                     RGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     misc_feature    15311..15327
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.86"
                     /tool="gutsmash"
     gene            15939..16598
                     /gene="radC"
                     /locus_tag="O9S30_RS05655"
                     /old_locus_tag="O9S30_05655"
     CDS             15939..16598
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /gene="radC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /locus_tag="O9S30_RS05655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05655"
                     /product="DNA repair protein RadC"
                     /protein_id="WP_009888913.1"
                     /transl_table=11
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANYI
                     INRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPWDI
                     QRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRSASK
                     IIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMII"
     misc_feature    complement(15970..15985)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 19.74"
                     /tool="gutsmash"
     gene            16668..17729
                     /locus_tag="O9S30_RS05660"
                     /old_locus_tag="O9S30_05660"
     CDS             16668..17729
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /GO_process="GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MreB_Mbl"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419052.1"
                     /locus_tag="O9S30_RS05660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05660"
                     /product="rod shape-determining protein"
                     /protein_id="WP_003428483.1"
                     /sec_met_domain="MreB_Mbl (E-value: 5.9e-155, bitscore:
                     512.8, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKEKKKEKKGFFSFNKMTKDMGIDLGTANTLVYIKGQGIVVREP
                     SVVAIRDDSKEVLAVGEEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAA
                     DKKGVVSPRIAICVPFGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPE
                     GNMVVDIGGGTSEIAVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENV
                     KINIGSTFKDDEEINMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLER
                     TPPELASDIMENGIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQ
                     EIFEKVLMMNTKN"
     gene            17739..18632
                     /gene="mreC"
                     /locus_tag="O9S30_RS05665"
                     /old_locus_tag="O9S30_05665"
     CDS             17739..18632
                     /GO_process="GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mreC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /locus_tag="O9S30_RS05665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05665"
                     /product="rod shape-determining protein MreC"
                     /protein_id="WP_009892910.1"
                     /transl_table=11
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGSP
                     VNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKDMIALN
                     AKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKKESI
                     VMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKDSYNS
                     KGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVVKPYVD
                     FKNINDVVVIEPRNIG"
     gene            18647..19135
                     /gene="mreD"
                     /locus_tag="O9S30_RS05670"
                     /old_locus_tag="O9S30_05670"
     CDS             18647..19135
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /GO_process="GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mreD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /locus_tag="O9S30_RS05670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05670"
                     /product="rod shape-determining protein MreD"
                     /protein_id="WP_003438053.1"
                     /transl_table=11
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLYL
                     DELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLIFI
                     GSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLKED"
     gene            19147..22125
                     /locus_tag="O9S30_RS05675"
                     /old_locus_tag="O9S30_05675"
     CDS             19147..22125
                     /GO_function="GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /locus_tag="O9S30_RS05675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05675"
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="WP_009888914.1"
                     /transl_table=11
                     /translation="MEENKQVDRLKIIKIVMSVIFLAILVKIIYMTTFKYEYYNELAEN
                     KTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKLIN
                     LLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKLISA
                     GILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKLSAKE
                     AFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTIAQIEE
                     SAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMVGLAGVE
                     KSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSDNALKQII
                     EVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDPNKFAKGIS
                     TEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMLTSMAALENGLDPNFTINDPG
                     VIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPDGKSIGIDMNA
                     NKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLEREMANDFVDIT
                     REKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKMNVKEDRIEEVADLAVFSYFNFA
                     KWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRAVSSDYSSVKINDQ
                     KKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKTGTAEKSGKIPTDNE
                     YEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKKLENKDLKDEERKKLE
                     EELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQDYGSFTWTVAFAPADD
                     PEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNKTNDVNNSSDENINFESQ
                     INR"
     gene            22223..22906
                     /locus_tag="O9S30_RS05680"
                     /old_locus_tag="O9S30_05680"
     CDS             22223..22906
                     /GO_process="GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /locus_tag="O9S30_RS05680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05680"
                     /product="septum site-determining protein MinC"
                     /protein_id="WP_009888915.1"
                     /transl_table=11
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEAY
                     VGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTLRS
                     GENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFVHAG
                     AEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYENESEISPEIAFVSNGRIVIESYL
                     SKLDK"
     gene            22930..23727
                     /gene="minD"
                     /locus_tag="O9S30_RS05685"
                     /old_locus_tag="O9S30_05685"
     CDS             22930..23727
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_process="GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="minD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) CbiA"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /locus_tag="O9S30_RS05685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05685"
                     /product="septum site-determining protein MinD"
                     /protein_id="WP_003419057.1"
                     /sec_met_domain="CbiA (E-value: 3.2e-23, bitscore: 80.0,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLRN
                     LDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQMID
                     LCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLEANE
                     IKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPAILDS
                     KSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            23745..24029
                     /gene="minE"
                     /locus_tag="O9S30_RS05690"
                     /old_locus_tag="O9S30_05690"
     CDS             23745..24029
                     /GO_process="GO:0000910 - cytokinesis [Evidence IEA]"
                     /GO_process="GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="minE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /locus_tag="O9S30_RS05690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O9S30_05690"
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="WP_003419058.1"
                     /transl_table=11
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKTD
                     ILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     misc_feature    complement(24641..24657)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 21.6"
                     /tool="gutsmash"
     misc_feature    complement(24717..24729)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 18.92"
                     /tool="gutsmash"
ORIGIN
        1 ttgtttgcaa catatttaat tttaatatac tattattatt atgggaatat taaataacac
       61 tatattggta tataatatat atgttacaaa aatagataat ttttaggagt gatataattt
      121 ggaaaaaaaa ttaattataa tagatggaaa ttcaataata aacagagcct tttatgctct
      181 gccagaaatg aataataaag aagggttaaa gaccaatgca atatatggat ttacaacaat
      241 gctatttaaa atgatagata tatataaacc aacacatata agtgtagcct ttgacagaaa
      301 agcacctaca tttagacatt tagaatataa agagtataag gctggaagaa agggaatgcc
      361 agatgaactg gcagaacaat tacagccatt aaaagacctt ttagataaat tcaaaataaa
      421 tagattggaa atagatgggt atgaggcaga cgacattata ggaactgttt ctaaaaaagc
      481 agaaaataat gggtataagg tatacatagt aactggagac aaagatgcta ttcaacttgc
      541 ttcagataat acaactactc ttataacaaa aaaaggagtt ggagaagttg aagaatacaa
      601 ctttaattct gtaattgaaa aatacgagat gactccagca caatttattg atttaaaagg
      661 tcttatggga gataagtcag ataatatacc tggagtacct ggaatcggtg aaaaaacagg
      721 tataaagctt ataaaagagt tttctagtat agagaattta atagagaata cagataagct
      781 taaaggtagt gtaaaaaaga aaatagagga aaataaagaa atagctattc aaagtaaaag
      841 acttgcaaca attataagag atgtgccaat agaagtggat ttagatagta tggtttttgg
      901 cgattatgat aaagatgaac ttttagataa atttagatac tttggattta caagtctctt
      961 atcaagatta gttgatttgg tcgattcaga tgacacagaa cacgtagaag aaaaaataga
     1021 aatattaaaa ttaaaagata cagaaaaatt tatagatgaa gtaaacaaaa aaggacaagt
     1081 tattttaaaa actgttagag gacaaggtaa tatacttgaa aaaaatataa tatatatttt
     1141 tataagtgta gatggagaaa aaatatatta tgtagattca aatgaagtgt caaagttaga
     1201 tagtatattt tcaaataatg aaattaaaaa gtatggatat aatttaaaag atgattatat
     1261 atctttaaaa ccatataata taaacttaga aaatatgtat tttgatataa caatagctga
     1321 gtacttaata gattcaactt catctacctc atatgattgt agtgcaatag ctatgaagta
     1381 tttatcaaaa aaggttaaat caaaagaaga attattagga aagggtgtta aatctaaaaa
     1441 ctatgaggat ttagaatttg aggatttagc agagtatatg ggtcaaataa tatgcacagt
     1501 taaagaaaca attcctatga tggaaagaag tctaatggat atgagtatgg atggtctttt
     1561 ttatcatgta gagatgcctt tagtagaagt cttaggtcat atggagtatg aaggtataaa
     1621 agtagacaaa agtatattgg atgaattgag tgtagagttt aaagaaataa tagctacact
     1681 agaaaaggaa atttatgaat tatcaggaga gccattcaat ataaattcac caaaacaatt
     1741 gggtgttata ttatttgaaa aattagagct tccaataatt aaaaaaacta aaactggata
     1801 ttcaactaat gcagatgttt tagaaaaatt aagagataag catccaataa tagataagat
     1861 aactgagtat agacaaatag taaagttaaa ttctacttac gttgaaggac ttttaaatat
     1921 aataaatcca gaaagtaata gaatacattc atcatttaat caaactataa cgactacagg
     1981 aagaatttca tctacagaac caaatcttca aaatatacct ataaaaatgg aaatgggtag
     2041 aaaaataaga aaagtgttta ttgcagatga taactgtaag ttggttgatg ccgattactc
     2101 acaagtagag ctaagagttc ttgcacatat gagtcaggat gaaaatatga tagaagcatt
     2161 taaacatcat gaagatattc ataccaaaac agcttcacaa gtttttaatg ttcctatgga
     2221 agatgttact agtgaattga gaggtgcagc taaagctgta aactttggta ttatatatgg
     2281 taaaagtgac tttggtctag ctgatgattt aaatatacct gttccaaagg cgaaagaata
     2341 tatcgaaagt tattttgcta aataccataa aataaaagag tttatggaca caacagtaga
     2401 aaaggctagt gaggatggat atgctgtaac aattttaaac agaagaagat atataccaga
     2461 aataaagtca agcaatttta tggaaagaaa tagaggaaag agatttgcaa tgaatgcacc
     2521 aatacaagga agtgcagcag atataataaa aattgctatg ataaatgttc ataaaaaatt
     2581 ggaagagaac aatttgaaat caaaacttat actacaagtt cacgacgagt tgatagttga
     2641 agcaattgat gatgaaatag atatagttaa gaaaatagta aaagaagaaa tggaaaatgc
     2701 tgtaaatatg gatgttcact tagatgtaga tttaaatgta ggtagttctt ggtatgagac
     2761 aaagtaggtg attttatgtt gatattagga cttacaggag gaataggctg tggaaaaagc
     2821 agcctatcaa atatatttag aaatcttaat atttctatag tggatgctga tattatatcc
     2881 aggaagatat ttgaggataa gttgttattg gaaaaagtat ttgtccattt tgggcaaagt
     2941 ataaaaaatg atgatggaac tctaaataga aaagctcttg ggaaaatagt ttttagcgat
     3001 gaagaaaaac ttaaggaatt aaatagcttg actcatccgc gaataagaga aaaaataata
     3061 agtgaaatag aagaattgag aaaaaaaggc gaaaatatag tagtattaga tgcagcaata
     3121 ttggtagaaa gtggtttttt agatatggtt gacaagctat tagtagttac ttgcaaacaa
     3181 gaagtacaaa ttagtagaat acaaaagaga gataattgta gtaagcagga agcacttagt
     3241 aggataaatt cacagatgag tcaggaagaa aaatccaaat atggtgatta tataatagat
     3301 aattcgggaa caataactga attagaaagt aaagcacata aatttattga gtacatgaag
     3361 gagaattggc gtgaatagta agaaagtatt gattctatct atttttataa tcttatttgg
     3421 ggcactatta atggaaagca aagtaataca taaattttta tatcctaaaa aatattcaga
     3481 gtatgtagaa aagtattcaa aagaatttaa tttagatgaa aatatagttt acagtgttat
     3541 taaagccgaa agtaagttta atagttctgc tgtttcaaaa aaggaagcaa aaggattaat
     3601 gcaaatatta gatataacta gagattgggg agcagaggaa ctaaatttaa aaaatgtgga
     3661 tattttcgac ccagagacta atataagact tggctgttgg tatttaagta agttatacaa
     3721 agaatttggt aaattagatt tagtgatagc tgcatataat ggtggttcag gtaatgtgaa
     3781 aaaatggtta gaaaataatg aatatagtaa agatggcgaa aatctacatg atataccttt
     3841 taagcaaact tcaaaatatg tagaaaaagt aaaaaataat tacgaacatt ataataagat
     3901 atatggcaag aaaggaaaaa actaatgaag agaataaaag tcttaacagt cgttttagct
     3961 ataactctta tggtagctgg ttgtagtaat actaaaacaa aacaaagtag tagtgattca
     4021 agtttacaaa gtcaaagttc tatagatgct aagtatataa acttaacaat ggtaactcca
     4081 aaaactataa atcctataac taatactgat aagtctgttg gttatataat gaatttagtt
     4141 tatgatagtt tatttactat agatgagaac tacaatgtaa ttcctcaatt agttaaggag
     4201 tataatatag ctcaggatgg aatgagtata gatataaagc taaaagatgc aaagtggcat
     4261 gatggaaaaa atgttacttc aaatgatgta aactatacta ttggtttaat tcaaaaaagt
     4321 gcagatagtc catataatga atttactaaa aatatagctt ctgtgaatat taaatcagat
     4381 aaagatttta caataaaatt taaagctaga tatgcttttt caattgatag tttaattttt
     4441 ccaatcgtat ctcaaaatca tttagattca aaggatgtaa atgacaagaa taataatatg
     4501 ataggaaatg ggaaatataa aatagaatca tatacagaaa gagaaggaat ggttttatct
     4561 gtaaacaaag attactatga agaagttcca aagaccatga agaacataaa agtaggtatg
     4621 gtgcctaatg aagaggcaag gacatctatg gttatggctc ttgatagtga tataaccaat
     4681 gttacattaa atgatttaag caagtttcaa gaaaaagagt ttaatataac aaaatatcaa
     4741 ggaagagatt atgagtgtgt attatttaat tataataatc cattttttaa agatgttaat
     4801 tttagaaaag caattgctca ctctatagat aaagatagaa ttattagtga aggttatatg
     4861 gatgatgcta ctccagttaa ttttccactt aattcaaaat caaaatacta taatagtgaa
     4921 atgaaggatt tagagtttaa taaagataag gcgatagagt gtcttgctaa ggtagaatat
     4981 gcaaatataa actcagtaaa tcaaaatgac aataaagtaa aaaatcagaa aaaatcagct
     5041 aaaaagaact caaagaagct tacgccagaa gaagaagcaa aagctaaaaa agcagaagaa
     5101 gatagaatca aaaaagaagc tgaaaagaaa aaaaatagag aaaaagagga agtaaagaaa
     5161 agtctttctg aaatgaactt aaagataata gtaaacagag ataatagtga gagaataaaa
     5221 actgcaaaca taataaatga aaacttaaaa gctataggaa taaatagtac agtaaatcag
     5281 ctgtcagata aggatatgga aaatgctctt aattctaaaa attatgattt agcattagtt
     5341 ggatggaaac tatcaattat accagatgca agtagtatta tagcaagtag tggatatact
     5401 gatgataagc taaatggata tatgtctgca cttacaagtt ctacgtctga aattgaaaca
     5461 aaaaaagcat acaaagatgt acagacatat ataaaagata atgcaacatt tataagttta
     5521 gctgtgagaa acaactatat tgtaagtaat agtagattaa aaggtaaaat tactccaaat
     5581 gattttgatg tatatgaggg aatatctaat ttagatataa aatcgaatga atcaaattaa
     5641 atgattaatt tctatttaaa aagtataaac tatatgatag aataccctta tggggtataa
     5701 ggaggtaatt aaatgaagaa gaaactatta gtagaaggta tgagttgtgg tcattgtgta
     5761 aaccatctta aaactgcatt aacagaagat atagatggta ttgaagtttt agatgtggat
     5821 ttagaaaata agtgtgcatc tgtagatatg aaagatgata tatctattga aaaattaaaa
     5881 gaagtgatag cagaattagg atttgaacta aagggaattg aatagaaatt gataaactaa
     5941 aaaaaggcct ctattggggg agtaatagag gtctaaaaat gggggagatt gagtatgttt
     6001 gattttacat tactattatc tcccctttta aattaatata tacataaaaa gtaaaaaaat
     6061 tttgtaatga ctcacatttc ttgagctttt tgaatatact ataaatagct aagggaggga
     6121 gtatcatgga atctagggga tataaagagg atttaaaaaa aatgatactt gatatggccc
     6181 acaaagaact tgagagttat gtatcacaaa aaggagcaaa aaaatacttt gaaggtaact
     6241 tggataagat tattaataat aatataaaaa aattcaatca aagtattggt cttaatagag
     6301 tatatttaaa aatgttaaaa aagtgtgcat atcaagaaag tgtaacttca atatcaagga
     6361 ctataaagaa agaagcatta tttaaatcaa aaaaagagct atttgctttt tcagagtact
     6421 taaaacttga tattaacaag cgactttcat ataatcaaat tttaagaaaa atatcaagat
     6481 atatatacta taatagaagt tcatatgctc aaaaatacgt tgttttcaaa agaggagatg
     6541 aagaatatct tttagaacca gagaaaataa aagatgaatt aatatcaagc tataggtcaa
     6601 agactagaga agatatgagg tcaatagcaa aacttttgga tatagaagta gaagatgatt
     6661 ataatgctga agatattaga aaaaaagtaa taaattatat tatcaaagaa aaacttaaaa
     6721 gttagagttt taaagaataa gttctgccaa tttgcatgat taagtataac ttgggtataa
     6781 attttttgtg catttgaaag agcttattct ttttttgaca tattctttta aataatttac
     6841 caccatttgg taattggtca tcatcattca aatttttaac ttaatatgat ttaaataaat
     6901 ttataataat gatataatat aaaaaaaata aaaattggag agtgatttca aaatgaagtt
     6961 taatttttgc cataacaatt tcaatgttac taacttagaa aaaagtttag atttttataa
     7021 aaaagcttta gggcttaaag aggtaaagcg taaggaagca gaagatggaa gttttatatt
     7081 agtttattta ggagatggta ttactagtca tacattagaa ttaacttggc ttagagattg
     7141 ggacagacca tataatttag gagacaatga atttcatctg gctttagaag ttgaggagtt
     7201 tgatgaggca aaaaaacttc ataaagattt agattgcata tgttttgaaa atgaaagtat
     7261 gggaatttat tttatagcgg acccagataa ttattggatt gaaatacttc ctaaaaatca
     7321 ttaattttaa ggatatgttt ataatttctt tacaattaat aagagaataa gttatcatta
     7381 aattgtaggg actaaaaatg gataataatg ttatgaattt atgactataa aaatttgttg
     7441 gggggagtga tagtagtgaa gaaagctata gcagctttag gaattggggc agtagcagta
     7501 tctgttagct ccataaatgc aagtgctctt gaaaagggaa cagtaacagc aagtgccttg
     7561 aatataagaa gtggaccaag ttctgattgt gataaggtag caaaattata caagggaaaa
     7621 actgtagaga ttttagaaaa gtctaatgga tggtataaag taagggtatc gagttctgtt
     7681 gttggatggg gaagcgcaaa atacatatca acaagtggtt catctgaagg tacatcaaat
     7741 caaaataatc caacttcaag tgggacaact attagtggaa atggtaaagt aaatgtgagt
     7801 tctagactga atgtaagaag tggtgcaggt actaactatt cattagttgg taaagcaaat
     7861 aatggagatg tagtaaagtt actagaacaa agcaatggat ggtataaaat aaaactatca
     7921 aatggagtta ctggatgggc tagtagccaa tatatctcta aaacatcaga ggatgttgga
     7981 acgaataatt ctagtaattc aaatagtact aataattctg ataaaaaacc atctagcgaa
     8041 gaatcaattg aaggcaagaa tggtaaggta acatctgctg tgagtttaaa tgtaagaagt
     8101 ggtcctggga caagctattc tataatagga aaattaaatg gtggagatgt tgttgaatta
     8161 aaatccaaaa ataatggttg gtataaggta aagttatcga gtggaacaat tggatgggtg
     8221 agtgccagtt atatttctga gactaatgaa ggtacaaaag aaaaaccaaa ttcatcatct
     8281 aaccaaaatt ctcaatctaa tagtaactca aagccatcat ttactggaaa ttcagataaa
     8341 tctacagcaa aaggttcaac aatcgtagat tttgcatata ctcttattgg aataccatat
     8401 caatggggag cttcaggacc tgataaattt gactgttctg gatttacaca gtatgttttt
     8461 aaacattctg taggcgtttc tattcctaga gtttctagag aacaggctaa ttttggaagt
     8521 gcaatttcta tggggaatta tgcaccagga gatttagttt attttgacac tgatggtgat
     8581 ggaacaacta atcatgtagg tatatatgta ggaaatagta aatttatcca ttgtagtggt
     8641 actcagacta atcctaataa ggttaaagtg gataatttaa ctagttcata ttggtctaaa
     8701 gtattacttg gtgcaagaag atttgtttag taagtacaat ttcttattaa aaaattagaa
     8761 gaaataatta tttatattca taaattgtga ctcttaaaaa tatactttat taataatata
     8821 attttgggag ggacaaacta atgaatagaa gaaaaacgat atacagagga tttaataata
     8881 atagacataa gattgattta cgtaaatatg ttataacgat tgcttgcttg tgtctaattg
     8941 gctattattc ttatacaaaa ataaaagata gcaagatatt ggagtatgta tcggctaaaa
     9001 taccatttct aaataattca tcagatataa cttataaaga tatatctgat gaattaaatt
     9061 caataaaaaa agggaaaaaa tctaaatcac aaaccaattc agatgataaa caagaaacta
     9121 atccagaaaa agcggtaaat aacactaaag agccagaaga agtcaaatta gctactatag
     9181 aaggctggga tatgtacact attcaagttg ctgcaataga taataatgat gatttgaaaa
     9241 aaatacagac atcattagtt aataatgaca tacctttttc agttatggag aaggatggag
     9301 taaaaaaaat acagacctat tcctcttttg atgaaaatga tgttagaaag cagataagca
     9361 gtgtaagaaa ggtgtttcca gatgcatttt tatcacactt agatgcacct atgttgtctt
     9421 tagagtatac aagtaactac gcttatatag agagtatatc taaagaatta aataaattaa
     9481 taacaaactt taaagaagaa tcaagttttt ggtcgaatgc tgaaaataat gtagatatgg
     9541 aaaaatataa tactattttg acaaatagaa aggcaatatc acaaaatata tcaaaagaag
     9601 ctgaaaagat tgactattct gaaatgcgtt tatttaaaga taatttaata gagtatgtaa
     9661 agaatgtgaa tgaaaaaatt gatacagctt caaaagcagc aaatgaagaa aaatatagtg
     9721 taagtaaaag tctgctttta tcatccatgc aggagtactc tatgtttata aattctataa
     9781 aataaaatac agaaaatttg gactttagaa agctatatga aaaggattgt ttaattaagc
     9841 aatccttttc ctcagtttga cttaatatag aggtattgat ttataaaata aattataaaa
     9901 atacggatat ttttttaaca attgttaaga ttttttaata tatatagtat acttatagaa
     9961 ggcagaaaaa atgttttttt atagataagg gggtaaaaca atgaaactct taacttttaa
    10021 gggagggata catcctccat atagaaaaga gtactctaac acaaaggcac ttgaaaaagc
    10081 tcaagctcca aagatagttt acattcctct tcagcaacat ataggggcac cagctaaacc
    10141 tattgttgaa gttggtgatg aagtaaagtt tgggcaaaaa ataggtgaac aacaaggttt
    10201 tgtttcatgt aacgttcatt cttcagtatc aggtaaagtt attgctattg aacaacatga
    10261 agtaccagga ggttcagctc aatgtgtagt tatcgaaaat gactttaagg aagaattaca
    10321 tgagagtgtt caaccaaaag gtcagttaga ggatttgagc aaagaagata tagtaggtat
    10381 cataaaagag gcaggaatag taggtatggg tggagctaca ttccctaacc atgtaaaagt
    10441 atctcctcca ccagatagca aggcagaagt agttatatta aatggtgcag aatgtgaacc
    10501 atacttaact gcagaccaca gattaatggt tgaaaatcca gaagatgttg tgtttggttt
    10561 aagagcatta atgaaggtat tagatgtcaa aaaaggattt ataggaattg aaacgaataa
    10621 accagatgct atagaagcaa ttcaaaatgt agctaaagac tatagtgaaa tcgaagttgt
    10681 tggccttcaa gtgaaatatc cacaaggagc agagaaacaa cttatttatg cgtgtacagg
    10741 caaagaagtt ccatctggtg gattaccaat agcagcaggt gcagttgttg acaatgttgc
    10801 cacagcagca caaatagcta agtctattaa aacaggtatg cctttggttg aaagaatcac
    10861 tacaataaca ggtagttgca tcaaagagcc aaagaaccta ataacaaaag ttggaacatt
    10921 agtttcagaa ataatagaac aatgtggtgg atttaaagaa gataagaaga ttggtaaggt
    10981 tataatgggt ggacctatga tgggaatagc tcaatatact actgaaatag cgactaacaa
    11041 aggttcttca ggaattttat gcttggatga ggaagaatca cgtactccag atattcaaaa
    11101 ctgtttaaga tgtggaagat gtacagatgt atgtccatca ttcttacaac cacttttcat
    11161 aagtgcatat tctttaaaag atgactatga cacagctgaa tatcatagag caatggattg
    11221 tatagaatgt gggtcttgtt catttatttg tccagcaaga agaccattac ttcaatctat
    11281 aagatcagca aagagagaaa taggagcaaa gagaagaaag caagctgctc agaaataaag
    11341 aaagttaagg gggaaaaacg atggaaaata agttgatagt atcatcttct cctcatgtga
    11401 gaagtaatga agatacttca tatataatga aacaagttat tatagcactc cttccagcag
    11461 cagtagcagg agtatacttc tttagactta atgctttgag tgctatgttt ttttgtatac
    11521 ttggtacagt aggtactgaa tttttatatc aaaaatttac gaagcaaaaa agtactatag
    11581 gagatttttc agctgttgta acaggattat tattagcatt taacgtacca gcatcacttc
    11641 cttggtggat gtgtctagta ggaggaatat ttgcaatatt agtagttaaa atggtatttg
    11701 gtggaattgg atgtaacttt gtcaatccag cacttgctgc aagagcattt ttattagcat
    11761 cattcccagt agcaatgact gcttggactc aaccaggtgt taactggata ggtaaaaatt
    11821 tagatgcagt tactacagca actccattaa gctttttgaa aaatggagca gcaggactgg
    11881 cagacctttc tagcaatgga attagtcttg ctgacatgat gatagggaat attggtggat
    11941 gtataggtga gacatcagca atattattat tattaggtgg agtataccta atgtataaag
    12001 gcataataaa ttatgttata ccagtatttt acatagcaac tgtatttata ttaacattcc
    12061 ttttaggtgg atttaatata actttcgcaa tatatcaact gtttgctgga ggacttatgt
    12121 taggtggatt cttcatgctt acggattata caacttcgcc tatgactaaa aaaggtcaaa
    12181 taatatacgc tgtattagct ggtcttataa caactgttat aagaatgtat ggtggatatc
    12241 cagaaggtgt atcttactca atactattag tgaactgtct tgctccactt atagataagt
    12301 ttgttagaaa tagagtattt ggggaggtgg ctaaataatg aacagtatgg tgagattagg
    12361 tggtacttta cttgctataa gtgcaatagc tgcattagct cttggagcta ctaatcaggt
    12421 tacagctcca gtaatagaac aaagaaatat acaagaaaat aatgaactta gaaaagcagt
    12481 ccttccagaa gcaaaagaat ttaaagaaat ggataaaagt acatataaag accttggaga
    12541 tggtcttata gctgaagtgt atgaaggttt agatggttct gaagtggttg gatatacttt
    12601 aaaagctaaa ccaagtggat atggtggaga aattgaagtt atggtaggta tatcatctga
    12661 aggtcaagtt acaggtgttg acattggtaa tatgtcagaa acagcaggtc ttggagcaaa
    12721 agctaaggat gatgcattta agggtcaata caaaggaaaa actgctgagc ctcttgaagt
    12781 agcaaaagga agtacaacag cagataatca aatattagca atatcaggtg caactattac
    12841 atctacagca gttactacag gagtaaatgc tgctatagat gttttcaata gtgctcttaa
    12901 taaataagga gggttaatta tgaatttagc taaagtattt aaaaatgggc taatagatga
    12961 gaacccaaca tttgttcaag ttataggtat gtgtccaaca ctagccgtta caacttcagc
    13021 aataaatgga attggtatgg gtctttcaac agcagcagtt ttaatatgtg caaatttagt
    13081 tatatcttta ataagaaaaa ttacaccaga taagataaga atacctatat ttatagtagt
    13141 tatagctaca tttgttacaa tagtaggtat gcttttaaaa gcatatgttc ctgctcttga
    13201 taaggctctt ggtatatata taccattaat agttgttaac tgtttaatat tagctcgtgc
    13261 agaaagtttt gctttcaaaa caggagctat gccttcaata gttgatggtg taggtcaagg
    13321 tcttggattt actgttgcac ttacaataat aggtgcagta agagaattac ttggaaatgg
    13381 tagtttattt ggaatgacat tatttggagc atctttccaa ccagtattaa tatttatatt
    13441 accaccaggt gcattcttaa cattaggatt cttatttgca ggatttaata aattaagaag
    13501 taaaaaagca tagtggaggt gtaataaatg aatttaatac ttttattttt aagtatcgtt
    13561 cttgttaata acgttataac atctcaattc ttgggtatat gtcctttctt gggagtttct
    13621 aagaaggttg atacagcagt agggatggga gttgcggtta cgttcgtttt aactctagct
    13681 tcaattataa cttattttat acagatttta ttagtaaaaa ctggaactgg attcttacaa
    13741 acaatagctt ttatattggt tatagcttct atagttcagt tcgttgaaat ggtaattcaa
    13801 aagatgagtc catctttata tcaagcatta ggagtttatt tacctcttat aactacaaac
    13861 tgtgcagttc ttggtatagc tctagttaat gttcaaaaaa gttataattt agtagaaaca
    13921 ataataaatg gatttggagc cggagctgga tttactttag caatagtaat attcgcaggt
    13981 ataagagaaa gactagagtt agctgatata ccagaagcat ttaaaggttt ccctataacg
    14041 cttatatcag caggtttaat gtcaatagct ttcttaggat ttacaggact tataaagcta
    14101 taaaaaagag gggtgaaaat ggtgatactt acagctgtat tagttttagg aattatgggc
    14161 ttaatcttcg ggatagtact agatttcgca tctaaaaagt ttgctgtaga agtagacgag
    14221 agagtggaag ctatactagg agtacttcca ggagccaact gtggaggttg tggatttcca
    14281 ggttgtggag gtcttgcaaa tgcgatagtt gaaggcaatg caccagttaa tggttgccct
    14341 gtaggtggag cagatgttgg tgcaaaagtt ggagaaataa tgggaataag tgctgaagca
    14401 ggtgagaaac aagtagcaaa agttatttgt aaaggaactt gttcaagtgc aaaagataaa
    14461 tatgagtatg aaggaatatc agattgtaga gcagcaaatg ttttaaactc aggtgctaag
    14521 atgtgtaaat ttggatgttt aggtcttgga acatgtaagg atgcttgtaa atttgatgct
    14581 atttctatag ttgatggaat agcagtaata gatgaagaaa agtgtgtaaa ttgtggaaaa
    14641 tgtaaagaag tttgtcctaa gggtataata atcacaaaac cagaaagtca agaagtagtt
    14701 gtagagtgta atagcaaaga gtttggtaaa gctgttaagg aaaaatgtac tgctggatgt
    14761 attggctgtg gaatgtgtgt aaaagcttgt aaatttgatg caataatatt tgaagacaaa
    14821 atagctaaga tagaccctaa taagtgtgta gggtgtatgc aatgtgtagc aaaatgccct
    14881 acaaaagtaa tatcaggtga cataactaag aaaaagaaag ttactattga ccaagaactt
    14941 tgtgttggat gtacagtgtg caaaaaacaa tgtaagtttg atgcaataga gggagaatta
    15001 aaagaaaaac ataaggtaga tgcagataaa tgtgtaggat gccatttatg tatggaaaaa
    15061 tgtccaaaga aagctattaa aattttgtaa tatggacaaa ataaggatat ggaataattt
    15121 ccatatctct aatgagtaca taaaacagag gtatggaata atttccatac ctctatttta
    15181 tgtatattta ttctaatggt aataaatata acaatagtgt aacaaaaaag gttcgacatt
    15241 tctgttgaat tttttacaat atagtgatag actaaatagg tacaaattac gtacaaatga
    15301 ggtgaataaa tgaatataat cttggcatca gcctctccaa gaaggaaaga aattctagag
    15361 aatacgaatg taagatttga tataattaaa aatgaaattg atgagattat attggaaggt
    15421 gaaacaccaa aacatttagt tatgagatta gcttttgaaa aaagtatgtc tgtagcatct
    15481 gaacataatg aagatgttgt tattggagct gatacagttg ttgttttaga caatgctatt
    15541 ttaggaaaac caaaagatga gtcttgtgca agagatatgt taaaaagact atctggaaga
    15601 gagcatcaag tcataacagg tattagctta ataaacttgt gcgaagataa aaaggtaatt
    15661 gactatgtaa tcagcaatgt gaaatttaaa acgttatcag agcaggatat tgaggattat
    15721 ctgaaaacta atgagtcatt tgacaaagca ggagcctatg gtatacaggg ttatggagct
    15781 ttgttagttg aagaaattag aggagattat tttaatatag ttggtcttcc aatttcaagg
    15841 ttaggcgacc tattaaaaaa atattttagt ataaatcttt tttatggagt gtgatttatc
    15901 tgaaaaagtc atttaattca accattaagg taaaagaaat ggcaccagag gaaagaccca
    15961 gagaaaagat gcttgccaaa ggcgtaaaaa gcttatcaaa tgctgagttg ttggctatac
    16021 ttttaagaac tggcaacaag aataaaaatg ctattgaatt agccaactat ataataaata
    16081 gagatattca gggtattaga catttagaag atatgacaat agaggagctg tgtaatatag
    16141 atggaattgg gttatcaaaa tcaacccaaa ttaaagcagc tttagaacta ggctctagag
    16201 tagctagttt taaacctata aagtataaaa taaggaatcc ttgggatata cagaggtatt
    16261 atatggatag cctaaggtat ttaaaaaaag aggtttttaa agcagttctt ttaaacacaa
    16321 aaaatgagat aatatctgat gtagatgtat ctatagggac tttaagctcg tcattagtcc
    16381 atccaagaga ggtttttaaa gaagctataa gaagaagtgc aagtaagata atagtgatgc
    16441 ataatcaccc ctcaggaagt gtagaaccat caagagaaga taaaaatatc acatcaagac
    16501 ttattaaatg tggagaaata attggaatag aaataataga ccatataatt attggagatg
    16561 gattatactt tagctttaaa gaaaatatga taatttgact ttatatgtga taaaataata
    16621 ggagaatgtt ttaaacatga ttcttaatag taggaaggag taaaatcatg gctaaggaaa
    16681 aaaagaaaga aaaaaaaggt ttttttagtt ttaataagat gacaaaggat atgggaatag
    16741 atttagggac tgcaaataca ttagtttaca taaaaggaca aggtattgtt gttagagaac
    16801 catcagttgt cgcaataaga gatgatagta aagaggtatt agcagtaggt gaagaagcca
    16861 aaaaaatgat aggtagaaca ccaggaaata tagtagctat aagacctatg aaagatggag
    16921 taatagctga ttttgacata actcaatcaa tgattagcta ttttatacaa aaagcggctg
    16981 ataaaaaagg tgttgtaagt ccaagaatag ctatatgtgt tccttttgga gttacagaag
    17041 tagaaaagag agctatagaa gaggctgcaa gacaagctgg agctaaagat gcattcctta
    17101 tagaagagcc aatggcagcg gctatagggg ctggattaaa agttgaagaa ccagaaggta
    17161 atatggtagt tgatattggt ggaggtactt cagaaatagc tgtaatatcc ttaggaggaa
    17221 tcgttactgc taaatctata agaataggtg gagacgaatt tgacgagtct atagtggctt
    17281 atgtaaaaaa agaatataat cttatgatag gtgaaagaac tgctgaaaat gtaaaaatta
    17341 acataggttc tacatttaaa gatgatgaag aaataaatat gcaaataaga ggtagagatt
    17401 taatctcagg attacctaaa acaatagaaa tatgctctac agaagttaga gaagcattaa
    17461 aagaacctgt aagttcaata gtagatgcaa taaaatctac actagaaaga actccaccag
    17521 aattagcctc agatataatg gaaaacggaa taatgctaac aggaggaggc gctttgctta
    17581 gaggtcttga taagcttatt acacaggaaa ctggtatgtc tgtacaaata gcagaaacac
    17641 ctcttgattg tgttgcactt ggaacaggta aatctgttga agaccaagaa atctttgaaa
    17701 aagtattaat gatgaatact aagaattaga agttggtgat ggccttgaga tttgacaaga
    17761 acaaaaatga gaaaaaaagg attaatgtta aagtgatagc aacgggcgta gttgctatca
    17821 ctttaattgg aattgtgggt atatcaattg gtaaattttc tagtggtagt ccagtaaatt
    17881 taggagtagc ttcagatgct attacaagtg taggtaaggg aataaatgat ggattttctt
    17941 ttatcaaaaa tggttttaaa gatgtagcaa attttaaaga taattctaaa aaggtaaaaa
    18001 aattggaaga agaaaatgaa aaacttaaaa aagatatgat agctttaaat gccaaactag
    18061 ataaaacaga atcgctagaa gagttaaaaa agacactgaa ctttgtacaa gaagagtata
    18121 aggcgacaag tatttcagca agtgttgttg gtaaaaatga tggaaactgg tatgagagtt
    18181 ttgtcatagg tgcaggaaaa aattcaggtg taaaaaaaga aagtatagtt atgaatggta
    18241 gtgggttagt tggcatagtt tatgaagttt ccaacaatta tagtaaggca atttcactat
    18301 tggattctaa ggcatctgta agctttaaac tagcaaagga tgccaatgct aaaggtacaa
    18361 ttactcaaaa tactacttta gataataaag atagttataa tagtaaagga tatttgcaag
    18421 gatatatgtt tgactcatca tataatgtaa ttcaaggaga tataataaca acatcaggtc
    18481 ttggattctt cccagatggt ataccaattg gcgaagttga aaaggtggtt gatgataaag
    18541 ataagtcatt aaaatatgta gttgtaaaac cttatgttga ttttaagaat ataaatgacg
    18601 ttgtagtcat agaaccaaga aatataggat aaaggggata aaaacaatga aaaaagtttt
    18661 actttgtctg ttggggattg tgcttatgac tatagaaaat agtataacaa attatataga
    18721 tatttttggc ataagcttta atttagttct tatatatgta accataatct ctctttattt
    18781 agatgaatta gaaggtggga ttatagcagc aataataggg cttattaaag acataaccgt
    18841 tggtggaatt tttggtgtaa atgcattaat tttgtttatt attgcatatg caattggata
    18901 catgagagat cagctatata aaggtagtta tattattact ttcgttttaa tttttatagg
    18961 cagtttaatt gattcaattg tcaatatagg tacatctagt ataatttatc aaagctacaa
    19021 catatcaaca ctttttgtaa aaggtctact agtagcacca atagtgaata gtttaattgg
    19081 tttagtaata tatcgcttat ctaaaagagc agttttaaaa cttaaagaag attagttggt
    19141 gatttcatgg aagaaaataa acaagttgat agactaaaaa taattaagat agtcatgagc
    19201 gtgatatttt tagctatttt agtaaaaata atctatatga ctacatttaa atatgaatat
    19261 tacaatgagt tagctgagaa taaaacatat aaaaagttgg caatagaagc tccaagagga
    19321 gaaataaaag atagatatgg aagactgttg gcaggaaata aaaacttatt tactgttcaa
    19381 gtttcaggta acgatattaa taagaaagat gcaaataagc attcaagagc aaatgaaata
    19441 tcattaaaac ttataaattt gctggagaga aatggtgaag aatatgtaga tgagtttcct
    19501 atatatgtag aaaatggaaa gtactattat acatatgaca gagatattag ggaatataaa
    19561 tcagaaaatg gtattccaaa tgattataat gctaaggaaa gtttttatta cttagtagac
    19621 aaacttatat cagctggaat attatctcaa gaagataaaa gattggatgc gacaagatta
    19681 caggcaaaac taaatgaaaa tggatattat ccacctattt tagttagtaa atggatgttc
    19741 acagcagaaa gagataagag agattggttg gctagttata agataaaaga aactaaactg
    19801 agtgccaaag aagcgtttga aaaagtaaga aatagtgatg cattagaaat tgataaaagt
    19861 ttgagtgatg aagatgcaag aaaaataatg gtcgtaaggg atttgataaa atcaaaagga
    19921 tattctcaat ataatccagt aactattgct aaggatgtag gagaaactac gattgctcaa
    19981 attgaagaaa gtgctatgga ccttgttggt gtttcaatag cagtagaacc agtaagatat
    20041 tatcctaatg ggtcattagc ttctcatatg ttaggatatg taggaaaaat gccatccact
    20101 caaatagaat cataccttca aaaaggttat gaaactggag atatggtagg tcttgcagga
    20161 gtagaaaaga gtaatgaaag tagattaaga ggaactgatg gttataaaat ggttaaagtt
    20221 gatgcattgg gtagaatcag taaagaaatt gaatctaaaa aacctaaatc aggagatact
    20281 gtgtatctta cactagataa agatttacaa gaagtttcag ataatgcatt aaaacagata
    20341 atcgaagttg cttctaaagg aggtactttt aaaagtaaat ttggggacaa accaattagt
    20401 gcatatgcag gaaaagcaca gtcagcagct cttatagcta ttgatgttaa aaatggagaa
    20461 gtattagctt catcaagtta tcctaattat gacccaaata aatttgcaaa aggtatttct
    20521 acagaggatt ataaagcact tcaaccaaaa aatccaaatg acttactagc aggaagtcca
    20581 cttttaaact tggttacaca aggggaattt cagccaggtt cttcatttaa gatgctaact
    20641 tcaatggcag cattagaaaa tggtttggat ccaaacttta ctataaatga ccctggggtt
    20701 ataatgttag gtaaaaaatc ttttggtgac tatgtatgga accatggtag aggaaaccat
    20761 ggtatgacaa acttatataa agcgatacaa gagtcttgta atatatatat ggcaacaatt
    20821 ggtacaggta aaacatggcc agatggaaaa agcattggta tagatatgaa tgcaaataaa
    20881 atacttgagt atgcaaaatt atttgggctt gaccaaaata cagggcttca agatgaagta
    20941 gaagaaagag caggtaaagt tcctagcaca gaagataaat tgaaatcaac acaagctctt
    21001 ctaaagtcaa atttagaaag agaaatggca aatgattttg ttgatataac tagagaaaaa
    21061 aatcctaaag aatatgagaa aagaatcaat gagattgtaa gctgggcagc agaaaagaaa
    21121 actcctggta gagtcgaaac aatgaatagg ctaaagaaaa tgaatgttaa agaagataga
    21181 atagaagaag tagcagattt agcagtattc agttatttta actttgcaaa atggagtact
    21241 gcagatacat ttaacttagc tataggtcaa ggtgaaaatg cttatacacc agctcaaata
    21301 tcaagatatg tagctgctat agcaaatgga ggtaatttag tagaattatc tgtagttgat
    21361 agagctgttt caagtgatta ttcatcagtt aaaattaatg accagaagaa agtcgaaaaa
    21421 atacctttta aaaatccaga taacttaaaa gaattaacaa aaggtatgaa attggttgca
    21481 agacaaggta cagcgaagag tgcttttgct gattttccaa tagatgtagc agcaaaaact
    21541 ggtacagctg agaaaagtgg gaaaatacct acagataacg agtatgaata tttaaaaagt
    21601 catatgtcat cttataatgt caacctaaat gatgcgatta aactagctga taagatgaaa
    21661 gcagaaaaag aaaaagagct ttcattagct aaggaaaagg aaataaaaaa gaaattagaa
    21721 aataaagatt tgaaagacga agaaagaaag aaattagagg aagaattaga agatggagta
    21781 aaagttagat tagaggacac tgataaggtg aattcttcat atctaagaaa agctataaaa
    21841 gaattaaatc caaaaataac agatgaccaa atagatagat ttaaacaaga ttatggttct
    21901 tttacatgga ctgttgcttt tgctccagca gatgacccag aaatagctgt tgtatgtgta
    21961 attcctcaag gtgattcaag tgtattctca cttttaccaa ctagagaagt tattggaact
    22021 tatatgggat tagagccaac taatagtaaa aatgacaaca agacgaatga tgttaacaat
    22081 tcttctgacg aaaatataaa tttcgagtca caaattaata gatagcttaa tctatgtata
    22141 aactttagct ttatatagaa aaaataaaag ggtttaaagc gtatttgaag aatataaatg
    22201 atatatcact ctggaggttg ttatgtcttt aagagaaatt tgtagtcaag aactagtaga
    22261 gttcaagggt aataaaagag ggataatagt aaatataaaa agagaagctc catttgagga
    22321 aatacaggaa aaaataataa acaaattgga agcatatgtt ggctttttta atggggctaa
    22381 aattagtaaa attaatagtg attgtttaac tgacatggag attctagaac ttaaagaagg
    22441 aattacttct agatttgatg tggaatttgt tgaagatcaa aaaattgaag aaaatagtaa
    22501 ttttccaaca aaatatgtca ataccctaag atctggtgaa aatattgaat ttgaaggtga
    22561 tgtagttata ttgaatgata tgaaacctgg ttcaaaagtc ctttcaaaat caaatacagt
    22621 tgttatggga gatataaatg ctggtgctaa ggttgtagca ggaggtaatg tttttgtaat
    22681 gggtaaaata gaaggttttg tacatgcagg tgcagaaggc aatgaatttg catatgttgt
    22741 tgctggaaat cttaatccta aaatattaca aatagcagat aatattgcag aagctccaga
    22801 tgatgaagaa aattatgaaa atgaatcaga aattagccca gaaatagcct ttgtaagtaa
    22861 tggaagaatt gtaatcgaaa gctatttgtc aaagttagac aaatagaaag aaaaataggg
    22921 ggtagtatta tgagcgaagt tatagttata acatctggta aaggtggggt tggaaaaact
    22981 actaccgcag caaacttagg aactgcgcta agcctagaaa ataaaaaaac agtagttgtg
    23041 gatgcagata ttggacttag aaacttagat gtagtaatgg gtcttgaaaa tcgaatagtt
    23101 tatgacatag tagatgtagt tgaaggaact tgtaggctta agcaagctct aataaaagac
    23161 aaaagatttg ataatctata tttattacca gcagcacaaa ctagagataa aaatgctgtc
    23221 tcagttgagc agatgattga tctgtgtgag aaactaaaag aatctttcga gtatataata
    23281 atagactgtc cagcaggtat tgagcaaggc tttaaaaatg cagtagctgg ggcagataga
    23341 gctattgtag ttactaatcc agaaatatca gcagtaagag atgctgatag aataataggt
    23401 ctattagaag caaatgaaat aaaagagata agattagtta taaatagaat tagaaatgat
    23461 atggttaagc gtggagacat gatggataaa caagatataa tagaaatatt agcaatagat
    23521 ttgttaggtc ttgttcctga tgatgaaagc ataattatat caacaaataa aggagagcca
    23581 gctatacttg attctaagtc acttgctggt caagcataca aaaatatcgc aaaaaggata
    23641 ctaaatgaag aagttcctct acttgatctt gaagttgaag atggattctt tggtagactt
    23701 aaaaaaatgt ttagcatggc taagtagcaa aggaggatac atctgtgtta gatttattta
    23761 gagttttttc taacgaggct aaaactagta aatctgttgc taaagagagg ttaaagctag
    23821 ttttagttca tgatagagta gattgttcac cacaactttt agagatgata aaaacagaca
    23881 ttttaaaagt aatagctaat tatgcagaaa tagaagatga tggtcttgaa ataaaaatgt
    23941 ctaaatgcag aggtgagcat gatgacaaac ctgtatctgc attagttgct aatataccac
    24001 taaagaatat aaaagataga tgtatgtaat tagtgcttaa aagggggata tccttattgg
    24061 tatatgcccc ttttatttaa ttatatgttt aaagatagaa agacggtgat tctttgaaaa
    24121 aaatcaatat taattataag tcgacaatta agttgataaa acagttagac tggaaactta
    24181 tagttacagt attagctatc tttatttttg ggcttgttat acttagtagt gcaacacatg
    24241 ctaattcaac aggttcatat aatcaattga ttaaacaagg attagctttt gtacttggta
    24301 taggaatgat tatagtcata ttattttttg actataatct tctaggcagg tattataaag
    24361 cgctttatat aataagtctt attttgttag ccatagtttt attacctgga attgggacag
    24421 taaaaggtgg agctaggtcg tggattaact taggaccact tgacttgcag acatctgaaa
    24481 tagtcaaatt aacatttgta ctaagttatg caaagatact tgaatccaaa aaggataaat
    24541 taaatacgtt aaaggaagtt atgcctgttg tagtttattc tcttccattt atcggattat
    24601 tgattgcaca accagacttg ggaacaggaa ttgtattttg ttgtatgata tttgcaatgc
    24661 tgttcacagc aggtcttagc tcaaagttga taaaaagagg aataatcata ctattagtgt
    24721 caatgccact gatgtattta atgatggcag accatcaaaa ggtaagaata gaagcttttc
    24781 taaatccaga agatgtaact ctaaaaggaa actatcaagt tatgcaatct ctgattgcaa
    24841 ttggttctgg gggcgtaact ggtaaaggac tttacaatgg aagtcaaaat caagaagatt
    24901 ttttacctgt acaagatagt gattttatat ttgctgttgt aggtgaagaa cttggtgtaa
    24961 taggaatggc agtgttgata atcttattta tgatattcct attaaggctt ttagcaattg
    25021 caagagatgc aaaagatttt tatggaaccc tcatagtggt tggtgttatg ggaatgtttg
    25081 gttaccagat
//
