LOCUS       FN545816               23126 bp    DNA     linear   BCT 27-FEB-2015
DEFINITION  Clostridium difficile R20291 complete genome.
ACCESSION   FN545816
VERSION     FN545816.1
KEYWORDS    .
SOURCE      Clostridioides difficile R20291 (Clostridium difficile R20291)
  ORGANISM  Clostridioides difficile R20291
            Bacteria; Firmicutes; Clostridia; Clostridiales;
            Peptostreptococcaceae; Clostridioides.
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-32bee55(changed)
            Run date     :: 2021-04-09 12:09:52
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 842067
            Orig. end    :: 865193
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..23126
                     /aStool="rule-based-clusters"
                     /contig_edge="False"
                     /core_location="[852067:855193]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      10001..13126
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..23126
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..23126
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="Pyruvate2acetate-formate"
                     /region_number="5"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     gene            475..1152
                     /locus_tag="CDR20291_0679"
     CDS             475..1152
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CDR20291_0679"
                     /db_xref="EnsemblGenomes-Tr:CBE02647"
                     /db_xref="GOA:C9YJC9"
                     /db_xref="InterPro:IPR000515"
                     /db_xref="InterPro:IPR010065"
                     /db_xref="UniProtKB/TrEMBL:C9YJC9"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) BPD_transp_1"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="CDR20291_0679"
                     /product="amino acid ABC transporter, permease protein"
                     /protein_id="CBE02647.1"
                     /sec_met_domain="BPD_transp_1 (E-value: 2.9e-25, bitscore:
                     86.8, seeds: 81, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAKI
                     SKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFALSV
                     NSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEFITL
                     VKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLEKKLS
                     VSN"
     gene            1211..1933
                     /locus_tag="CDR20291_0680"
     CDS             1211..1933
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CDR20291_0680"
                     /db_xref="EnsemblGenomes-Tr:CBE02649"
                     /db_xref="GOA:C9YJD0"
                     /db_xref="InterPro:IPR003439"
                     /db_xref="InterPro:IPR003593"
                     /db_xref="InterPro:IPR017871"
                     /db_xref="InterPro:IPR027417"
                     /db_xref="UniProtKB/TrEMBL:C9YJD0"
                     /locus_tag="CDR20291_0680"
                     /product="amino acid ABC transporter, ATP-binding protein"
                     /protein_id="CBE02649.1"
                     /transl_table=11
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLLR
                     CMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAPIK
                     LKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLFDEP
                     TSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDNTPEE
                     VFGNPKHERTKAFLAKVL"
     gene            2581..3732
                     /gene="iscS1"
                     /locus_tag="CDR20291_0681"
     CDS             2581..3732
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CDR20291_0681"
                     /db_xref="EnsemblGenomes-Tr:CBE02650"
                     /db_xref="GOA:C9YJD1"
                     /db_xref="InterPro:IPR000192"
                     /db_xref="InterPro:IPR015421"
                     /db_xref="InterPro:IPR015422"
                     /db_xref="InterPro:IPR015424"
                     /db_xref="InterPro:IPR016454"
                     /db_xref="InterPro:IPR020578"
                     /db_xref="UniProtKB/TrEMBL:C9YJD1"
                     /gene="iscS1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="CDR20291_0681"
                     /product="putative cysteine desulfurase"
                     /protein_id="CBE02650.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 3.6e-07, bitscore:
                     27.4, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_5 (E-value: 2.5e-83, bitscore:
                     277.8, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEKA
                     IKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSVLN
                     TLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGEVGK
                     YLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKENNRLK
                     PMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDVVISKVNNLKNILKNEIIDNIE
                     DIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYVLNAIGL
                     TNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            3778..4953
                     /gene="thiI"
                     /locus_tag="CDR20291_0682"
     CDS             3778..4953
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CDR20291_0682"
                     /db_xref="EnsemblGenomes-Tr:CBE02652"
                     /db_xref="GOA:C9YJD2"
                     /db_xref="InterPro:IPR003720"
                     /db_xref="InterPro:IPR004114"
                     /db_xref="InterPro:IPR014729"
                     /db_xref="InterPro:IPR020536"
                     /db_xref="UniProtKB/TrEMBL:C9YJD2"
                     /gene="thiI"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ThiI"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="CDR20291_0682"
                     /product="putative thiamine biosynthesis protein"
                     /protein_id="CBE02652.1"
                     /sec_met_domain="ThiI (E-value: 5.7e-71, bitscore: 235.9,
                     seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVYK
                     EYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEAGY
                     KTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFHTMV
                     YSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTSERSQ
                     EKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQRLSEKR
                     HCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIGTFETSI
                     VPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            5094..5912
                     /locus_tag="CDR20291_0683"
     CDS             5094..5912
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CDR20291_0683"
                     /db_xref="EnsemblGenomes-Tr:CBE02653"
                     /db_xref="GOA:C9YJD3"
                     /db_xref="InterPro:IPR012902"
                     /db_xref="PDB:4IXJ"
                     /db_xref="UniProtKB/TrEMBL:C9YJD3"
                     /locus_tag="CDR20291_0683"
                     /product="fimbrial protein (pilin)"
                     /protein_id="CBE02653.1"
                     /transl_table=11
                     /translation="MGMIIMNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVT
                     CLSNRENIKTQIVIAMAEESSKDKNEVIKEVLENKDGKYFETEPKCKSGGIYSATFDDG
                     YDGITGIESIAKVYVTCTKHPDGIEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFR
                     KYLLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWY
                     TSLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            6155..6796
                     /locus_tag="CDR20291_0684"
     CDS             6155..6796
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CDR20291_0684"
                     /db_xref="EnsemblGenomes-Tr:CBE02655"
                     /db_xref="GOA:C9YJD4"
                     /db_xref="InterPro:IPR000415"
                     /db_xref="InterPro:IPR029479"
                     /db_xref="UniProtKB/TrEMBL:C9YJD4"
                     /locus_tag="CDR20291_0684"
                     /product="putative reductase"
                     /protein_id="CBE02655.1"
                     /transl_table=11
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAPS
                     GCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDESS
                     SWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVKESE
                     SIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
     gene            8209..9879
                     /locus_tag="CDR20291_0685"
     CDS             8209..9879
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CDR20291_0685"
                     /db_xref="EnsemblGenomes-Tr:CBE02656"
                     /db_xref="GOA:C9YJD5"
                     /db_xref="InterPro:IPR000014"
                     /db_xref="InterPro:IPR000160"
                     /db_xref="InterPro:IPR001610"
                     /db_xref="InterPro:IPR001633"
                     /db_xref="InterPro:IPR013655"
                     /db_xref="InterPro:IPR029787"
                     /db_xref="UniProtKB/TrEMBL:C9YJD5"
                     /locus_tag="CDR20291_0685"
                     /product="putative signaling protein"
                     /protein_id="CBE02656.1"
                     /transl_table=11
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAIF
                     DEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYEEIVW
                     ISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIYKNG
                     AMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFYPMCT
                     KETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIALDIAK
                     ISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKEVIGAEV
                     LLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDFKISVNVS
                     YIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLKGIGVYIAI
                     DDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHIINLKVCVEG
                     IESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(10001..10735)
                     /gene="plfA"
                     /locus_tag="CDR20291_0686"
     CDS             complement(10001..10735)
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CDR20291_0686"
                     /db_xref="EnsemblGenomes-Tr:CBE02658"
                     /db_xref="GOA:C9YJD6"
                     /db_xref="InterPro:IPR001989"
                     /db_xref="InterPro:IPR007197"
                     /db_xref="InterPro:IPR012838"
                     /db_xref="UniProtKB/TrEMBL:C9YJD6"
                     /gene="plfA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_kind="biosynthetic"
                     /locus_tag="CDR20291_0686"
                     /product="pyruvate formate-lyase activating enzyme"
                     /protein_id="CBE02658.1"
                     /sec_met_domain="Radical_SAM (E-value: 2.5e-27, bitscore:
                     94.0, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDIK
                     SGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTCLD
                     TSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIPVWI
                     RHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKDVPDA
                     TKEDVQKASEIISKFGVKVYNS"
     gene            complement(10895..13126)
                     /gene="plfB"
                     /locus_tag="CDR20291_0687"
     CDS             complement(10895..13126)
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CDR20291_0687"
                     /db_xref="EnsemblGenomes-Tr:CBE02660"
                     /db_xref="GOA:C9YJD7"
                     /db_xref="InterPro:IPR001150"
                     /db_xref="InterPro:IPR004184"
                     /db_xref="InterPro:IPR005949"
                     /db_xref="InterPro:IPR019777"
                     /db_xref="UniProtKB/TrEMBL:C9YJD7"
                     /gene="plfB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="CDR20291_0687"
                     /product="formate acetyltransferase"
                     /protein_id="CBE02660.1"
                     /sec_met_domain="Gly_radical (E-value: 8.3e-46, bitscore:
                     152.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 9.7e-294, bitscore:
                     973.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTKK
                     LWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAVMP
                     YGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITGLPD
                     AYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISALNEL
                     KKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYFERDLK
                     AGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDGRTLVTK
                     NSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDDLMRAYWG
                     DDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRFEPITSEYL
                     DYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFRTMACGMAGL
                     SVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAVELVESFMNKI
                     RKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPMHGRDTNGALAS
                     LSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFSQNAHHLNVNVFD
                     RATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHGKMA"
     gene            13419..14480
                     /locus_tag="CDR20291_0688"
     CDS             13419..14480
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CDR20291_0688"
                     /db_xref="EnsemblGenomes-Tr:CBE02661"
                     /db_xref="GOA:C9YJD8"
                     /db_xref="InterPro:IPR004481"
                     /db_xref="InterPro:IPR004837"
                     /db_xref="UniProtKB/TrEMBL:C9YJD8"
                     /locus_tag="CDR20291_0688"
                     /product="Ca2+/Na+ antiporter"
                     /protein_id="CBE02661.1"
                     /transl_table=11
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIFI
                     NCTVEIGRKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICNTG
                     LALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIFYMV
                     NNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMMIIGS
                     QILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNILGANI
                     LNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNRLSRIQGILMLFTY
                     FLYISILYFMYII"
     gene            14571..16184
                     /locus_tag="CDR20291_0689"
     CDS             14571..16184
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CDR20291_0689"
                     /db_xref="EnsemblGenomes-Tr:CBE02663"
                     /db_xref="GOA:C9YJD9"
                     /db_xref="InterPro:IPR000629"
                     /db_xref="InterPro:IPR001650"
                     /db_xref="InterPro:IPR005580"
                     /db_xref="InterPro:IPR011545"
                     /db_xref="InterPro:IPR014001"
                     /db_xref="InterPro:IPR014014"
                     /db_xref="InterPro:IPR027417"
                     /db_xref="UniProtKB/TrEMBL:C9YJD9"
                     /locus_tag="CDR20291_0689"
                     /product="putative ATP-dependent RNA helicase"
                     /protein_id="CBE02663.1"
                     /transl_table=11
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDVI
                     GQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGIKT
                     LPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEMLDMG
                     FREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNTKQYY
                     IETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGDLKQTQ
                     RDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRTGRAGRE
                     GMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQTIEEGHLT
                     KQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKGGTGAKDGM
                     IRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKKDAKTVIEKM
                     KDIKIKGNKINIEKANKKKK"
     gene            16459..18444
                     /locus_tag="CDR20291_0690"
     CDS             16459..18444
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CDR20291_0690"
                     /db_xref="EnsemblGenomes-Tr:CBE02665"
                     /db_xref="GOA:C9YJE0"
                     /db_xref="InterPro:IPR001034"
                     /db_xref="InterPro:IPR002178"
                     /db_xref="InterPro:IPR003501"
                     /db_xref="InterPro:IPR007737"
                     /db_xref="InterPro:IPR011608"
                     /db_xref="InterPro:IPR011991"
                     /db_xref="InterPro:IPR013011"
                     /db_xref="InterPro:IPR013196"
                     /db_xref="InterPro:IPR016152"
                     /db_xref="UniProtKB/TrEMBL:C9YJE0"
                     /locus_tag="CDR20291_0690"
                     /product="putative transcription antiterminator"
                     /protein_id="CBE02665.1"
                     /transl_table=11
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYINQ
                     INRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYVLD
                     ILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGEEMD
                     IRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLRDVER
                     YIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKETIFLTM
                     PLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLEYHLYFA
                     LNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYIALHFGSY
                     FEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAKANLDLLKY
                     YDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIKKIEKNIPFI
                     GKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKSSTALDNYIAL
                     PHSVNTNGENLYLSFGILEKPVVWDTKEIRIIILMIIPDKNVDSTDLIIKSYEEVLEVG
                     RDKKMIEELAKVKSFDEFYKIITKRR"
     gene            18449..18883
                     /gene="gutM"
                     /locus_tag="CDR20291_0691"
     CDS             18449..18883
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CDR20291_0691"
                     /db_xref="EnsemblGenomes-Tr:CBE02667"
                     /db_xref="InterPro:IPR009693"
                     /db_xref="UniProtKB/TrEMBL:C9YJE1"
                     /gene="gutM"
                     /locus_tag="CDR20291_0691"
                     /product="glucitol operon activator protein"
                     /protein_id="CBE02667.1"
                     /transl_table=11
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAIG
                     RKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLTVY
                     NKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            18912..19472
                     /gene="gutA"
                     /locus_tag="CDR20291_0692"
     CDS             18912..19472
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CDR20291_0692"
                     /db_xref="EnsemblGenomes-Tr:CBE02669"
                     /db_xref="GOA:C9YJE2"
                     /db_xref="InterPro:IPR004699"
                     /db_xref="UniProtKB/TrEMBL:C9YJE2"
                     /gene="gutA"
                     /locus_tag="CDR20291_0692"
                     /product="PTS system, glucitol/sorbitol-specific IIc2
                     component"
                     /protein_id="CBE02669.1"
                     /transl_table=11
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTMN
                     AIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSYFA
                     AASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIKGVV
                     TDYTTKFVEKQQGIALNSDIKIG"
     gene            19536..19904
                     /gene="srlE"
                     /locus_tag="CDR20291_0693"
     CDS             19536..19904
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CDR20291_0693"
                     /db_xref="EnsemblGenomes-Tr:CBE02670"
                     /db_xref="GOA:C9YJE3"
                     /db_xref="InterPro:IPR011618"
                     /db_xref="UniProtKB/TrEMBL:C9YJE3"
                     /gene="srlE"
                     /locus_tag="CDR20291_0693"
                     /product="PTS system, glucitol/sorbitol-specific IIb
                     component"
                     /protein_id="CBE02670.1"
                     /transl_table=11
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVKI
                     AELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLAMF
                     IKEDIYVSGVKPTNVEQL"
     gene            19929..20495
                     /gene="srlE'"
                     /locus_tag="CDR20291_0694"
     CDS             19929..20495
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CDR20291_0694"
                     /db_xref="EnsemblGenomes-Tr:CBE02672"
                     /db_xref="GOA:C9YJE4"
                     /db_xref="InterPro:IPR011618"
                     /db_xref="InterPro:IPR011638"
                     /db_xref="UniProtKB/TrEMBL:C9YJE4"
                     /gene="srlE_"
                     /locus_tag="CDR20291_0694"
                     /product="PTS system, glucitol/sorbitol-specific IIc
                     component"
                     /protein_id="CBE02672.1"
                     /transl_table=11
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIHT
                     ILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPGAV
                     IAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVGVPS
                     VLYSRFLTGAPTVLLAWLASFGLYK"
     gene            20536..20901
                     /gene="srlB"
                     /locus_tag="CDR20291_0695"
     CDS             20536..20901
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CDR20291_0695"
                     /db_xref="EnsemblGenomes-Tr:CBE02674"
                     /db_xref="GOA:C9YJE5"
                     /db_xref="InterPro:IPR004716"
                     /db_xref="UniProtKB/TrEMBL:C9YJE5"
                     /gene="srlB"
                     /locus_tag="CDR20291_0695"
                     /product="PTS system, glucitol/sorbitol-specific IIa
                     component"
                     /protein_id="CBE02674.1"
                     /transl_table=11
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHSE
                     NNLVKEILTNDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYLEE
                     KEIAPIGVGTILKIIRK"
     gene            20996..21775
                     /gene="gutD"
                     /locus_tag="CDR20291_0696"
     CDS             20996..21775
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CDR20291_0696"
                     /db_xref="EnsemblGenomes-Tr:CBE02676"
                     /db_xref="GOA:C9YJE6"
                     /db_xref="InterPro:IPR002198"
                     /db_xref="InterPro:IPR002347"
                     /db_xref="InterPro:IPR016040"
                     /db_xref="InterPro:IPR020904"
                     /db_xref="UniProtKB/TrEMBL:C9YJE6"
                     /gene="gutD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="CDR20291_0696"
                     /product="sorbitol-6-phosphate 2-dehydrogenase"
                     /protein_id="CBE02676.1"
                     /sec_met_domain="adh_short (E-value: 2.1e-47, bitscore:
                     158.8, seeds: 44, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKMRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVA
                     EEIETEFGCKSVGYGCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAGV
                     GLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKVPL
                     KRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
ORIGIN
        1 ttatattatt taagtagaaa tgcagttata gttaaagatg cagatataga caaagtaaaa
       61 actgaagatg atttaaagaa acttagagtt ggagttcaag caggaagtat ccaagaagaa
      121 tatgtagtta acactttaaa aatgacaact acaaaatctt taaaagcaat acctgattta
      181 attacagaat taaaaaatgg aaacatagat gcagttgtta ctaatgaagc agtttcttta
      241 ataaatgtta aaaaatatga tggaataaaa atggcaaata ctgaagttgg taaagatgta
      301 acagaaggta tggcagcagc aatcaaaaaa tctgacaata acaaagattt tatagaatta
      361 ttaaacaaaa agataaaaga attacaagat ggtaagaaga tagaagaatt tttaaatgaa
      421 gcatctactg aagctgcttc aaattagtaa gaaagttatg tgggagatga aattatgagt
      481 ttagatttta gttttttaag tagatttggg acttcttttt tggaaggaac aggtgtgaca
      541 gtatcaattt ctcttgtggc attatgcttt ggatttataa taggtataat tatctgtatg
      601 gcaaaaatat caaagagcaa agtattaaga gcaatatcat caatctatat tgaggtttta
      661 agagggacac cattattagt acagatatat atagtatggt ttggtttacc tcaattagga
      721 ataagattcc ctatgttgtt tggtatacca agtgaattta tagctagtgc atttgcatta
      781 tcagtaaact ctggtgcata cgtggcagag atacttagat caggtataca atcagttgat
      841 aatggacaaa tggaagcaag tagatcatta ggattaaatt attggtcaac tatgagatat
      901 ataataatac ctcaagctat aaaaaatata ttaccttcat tagcaaatga gtttattaca
      961 cttgtaaaag aatcttcaat aatatctgta ataggagttg ttgagataat gcgtactgct
     1021 gatatagtaa aaaatgcagc atttagagca ttagagccat taatagtagc agcagcaatt
     1081 tactttgtta taacatttac tctatcaaga ttagttggat tattagaaaa gaaactatct
     1141 gtgtctaact agatgagagc atgtttagca tttagggaaa acatagaata gtaaaaagga
     1201 aagtgataat atgattacaa taaaaaattt aagtaaatct tttggagatt taaatgtatt
     1261 aaaaaatata gacttagaaa tcgctaaagg cgaaataatg gttatagtag gtccaagtgg
     1321 ttctggtaaa agtacacttc ttagatgtat gaatttacta gaaataccta caggtggaga
     1381 aataatattt gaaggaaaaa atttggtcga taaaaaaact aatattgatg aggttagaca
     1441 aaatataggt atggtgtttc aaaactttaa tttattccca cataagacta ttttagataa
     1501 tataacatta gcaccaatta aattaaaaaa gatgactaag gaagaagcag aaaaaaaagc
     1561 agaaattttg ttgagtagag ttggtctttt agataaaaaa gattcatatc catcacaatt
     1621 atctggagga caaaagcaaa gaatagcaat agctagagca cttgctatgg aaccagatat
     1681 gatgttgttt gatgagccaa catctgctct tgacccagaa atggtaaacg aagttttgga
     1741 tgttataaaa gaacttgcta aagaaggtat gacaatggca attgtaactc atgagatggg
     1801 atttgctaag gaagttgcag atagagttat ttttatagat ggaggaagta tacttgaaga
     1861 taatactcca gaagaagttt tcggaaatcc aaaacatgaa agaacaaaag catttttagc
     1921 aaaagtgcta taatatataa aaaggatgtt attgatatta gaaattcaat acatcctttt
     1981 ttagtgatgg ttaatattaa ttttaaaatt aaagttatat taatattaac tttattttta
     2041 cgattttttg cagaaaaaaa aagagctcgg ggtaagctct ttttttccac cgaattaatt
     2101 tccgttacta atccgatttt taataaatat aaaatatatc cttatgaaag ggtattggga
     2161 atagatatat taactaacta gggttaccag ggggataacc ttaatttatt ataacaaaat
     2221 gcgacacaaa aatcaatatt ttttttgaaa taaattataa aagttatttt tataacaagt
     2281 aagcttataa atttaacttt tatagtaaaa ataagtatat gtataatata atatatttat
     2341 tttatataat atattatttt atcatatatt gtattttata atatattttt gtaaggtata
     2401 gtataagtat attatatttt actataaaaa tttgtaataa agtgaaaaaa ctagcttttc
     2461 atgaaataat attttataat ttaagattgt ctttttaata aagattaaca aaaaaaatac
     2521 tataattagt ggtataatag ttagagttga agattttaaa taaaggtagg tgtaattaag
     2581 ttggaaatat acttagacaa tagcgcaact acaaaacctt atcaagaagt tatagataaa
     2641 atggtttatg cacttaatac ggaatatgga aatccttctt ctgtccatag aaaaggtgta
     2701 gaagttgaaa aagctataaa agaagtaaga caagatatag ctaaatctct aggagcgaaa
     2761 gaaaaggaga tttattttac atcaggtggt acagagtgta ataatactat aataagaggt
     2821 attactagtt taaataaaaa aagaaaaaat cacataatat ctactaacat agaacatcct
     2881 tctgttttaa atacattaaa agatttagaa gaagatggtt ttgaagtaac ttatttagag
     2941 gtcggaaaag atgggaaaat aaatatagag gatttaaaaa atgcgataaa gtcaactaca
     3001 tgcttagtaa gcatgatgca tgttaacaat gaaataggaa ctatacagcc aataggtgaa
     3061 gttggaaagt atttgaaggg tttaaaagaa aaaatttatt tccatgttga tgctattcag
     3121 tcatatggaa aaattaattt tagaccatct aaatacaata tagattttat gagtgtaagt
     3181 gcacataaat ttcatggtcc aaagggaatt ggatttatgt atataaaaga aaataatcgc
     3241 cttaagccta tgttaacagg tggtggtcag gaaattggaa ttaggtcggg aacagaaaat
     3301 gtacctggaa tatatggtct tggagaagct gttagaatac taaacaaaga tttagatgtt
     3361 gttatttcga aagtaaataa cttaaaaaat atattaaaaa atgaaataat tgataatata
     3421 gaagatataa aaatcaattc tccagaagat ggagtatgtc atatattaaa tgtatcattt
     3481 agagggacaa aaggagaagt tttacttcat tacctagagc aaaaaggtat ttatgtatca
     3541 actggctctg catgttcttc taaaaagaaa ggtagttacg tattaaatgc tataggtctt
     3601 acaaatgaag aaataaatgg aacaataaga tttagtttat ctgatatgaa tacagaagaa
     3661 gaaatgttag aagctgtgaa agttttgaaa gaatcaattt gtgacttaag gtcaataatg
     3721 aaaagaaaat aaagaatact gtatagatag tgataataaa ttagtttaag aggtgaaatg
     3781 gtgtataata tattaatagt aaagtatgga gaaattggag taaaaggaaa gaatagatat
     3841 atatttgaga ataggcttat aagaaatata agaaatatgt taaaaccaat tggaagattt
     3901 aatgtatata aagagtatgg aagaatatat gttgatttag aagactatga ttatgaagaa
     3961 gtaattgaag aagttagaaa agtatttggg atagtgggtg tgtgccctgg agtaagagct
     4021 aaaaaagatt atgatacatt aaaagaaata gctttaaaaa tgttagaaga aaaaatagag
     4081 gctggatata aaacttttaa ggtagagtca agaagaggag ataagtcttt cgggttgact
     4141 tctcaagaaa tgagtatgga tataggtgga tacttattat caaaggtagg agataggatt
     4201 aatgttgatg taagaaatcc agaagttaaa ataaagtgcg aatacagaga atttcatact
     4261 atggtctata gtgatactat acctggatat ggtggattgc cacttggaac gaatggtaga
     4321 gctatgtctc ttttatcagg aggtatagat agtccagtag ctacatggat ggttgcaaaa
     4381 agaggtatgg aggtagaggc agttcacttc catagttatc catttacaag tgaaaggtca
     4441 caggaaaaag ttaaggattt agctaaaata ctggcaaaat actgtggtag agtaagactt
     4501 cataaagtta acatattaga aatacaaaaa gcaattggtg agaattgtaa tgaagaagaa
     4561 gctactattc tttcaagaag atttatgatg aggatagcac agagactttc tgaaaaaaga
     4621 cattgtgatg ctttaattac aggtgaaagt attgggcagg ttgcatctca aactatacaa
     4681 gggcttactt gtactaatgc cgttgtagac ttaccagtgt ttagaccact tatagcaatg
     4741 gataaatcag atatagttga tatagccaaa aaaataggga cttttgaaac atctatagtg
     4801 cctgaagaag attgttgtag tgtattttct cctagaaagc ctgtcactaa acctagatta
     4861 gagaaaatag aaaaatctga aacagcactt gatattgaaa aattagtaca ggatgctatt
     4921 gacaaaatag aggtagagga tattgagttt tagcatacaa gttatttaat tactaaattg
     4981 aatattgagt atattagtag ttctgtaagt atattagtag ttctgttgaa aggatttatg
     5041 tagacaattt attaatatat agtatttaaa aataagtact ataattttat ttaatgggaa
     5101 tgattattat gaataaaaag ggttttacat taattgaatt gttggtagtt atatctataa
     5161 taggaatttt agttatagta gctgttccag cgttatttag aaatatagaa aaaagtaagg
     5221 cagttacatg tctttctaat agagaaaata taaagactca aattgttatt gcaatggctg
     5281 aggaatcaag taaagacaag aatgaagtca taaaagaggt attagaaaac aaagatggta
     5341 agtactttga aacagaacca aagtgtaagt caggtggaat atattcagca acgtttgatg
     5401 atggttatga tggaataact ggaatagaaa gcattgcaaa agtgtatgtt acttgtacaa
     5461 aacatccaga tggtattgaa atggctaggg atatacatca aagtatgaaa gatttgattg
     5521 catcatttgc acaagaccct tctataatac caggagcttc aaagggcaat gatgatttta
     5581 gaaaatattt attagacaat aaatataaaa atgggtggcc tacaattcca gatgaattta
     5641 aggcaaaata tggattaagt aaggatacac tatatataca accatatgca tataatccta
     5701 ctaaatctga tgctactgta gttgtatttg caaataataa gactggaggt aattggtata
     5761 cttccctagt ttacgattat gatgaaggta gatggtataa aggtaaaaat ggtatttctg
     5821 ttgcaggtag gtcatgggat gttgacacag atagtgttaa gtctgtaaaa acagagattc
     5881 attctaaaga gggatggggt cctttaaatt aatatatcat gttttattaa tctggtattg
     5941 attaaactat agaaaagagc agtttctata gatgacgaat accagatttt catttatatg
     6001 tataattaag atatattaaa agtatttata tttttaagtc ttcacataca agctaaagat
     6061 aattataata atattgtaaa gaaatttaat tataagatta attttctgaa gatttagtat
     6121 ttgtactaag aaatacataa aaaggggaat agatatgaat aataattttc aagacaacca
     6181 aacaataaat ttaatacaaa gtcgtagaag tatacgaaaa tttacaacag agcaaattag
     6241 tgatgagcaa gtaaacacat tattacactg tgcatttgca gcaccttcag gatgtaataa
     6301 acagccttgg cacataactg ttgtgcaaga ccagaaactt ttaaaagaga ttagtgatga
     6361 tactttatct aggattcatg aagtgagtaa tgttgagata aataaaaact ttaagttatt
     6421 ttatggtgct ccaaccgttt tatttatttc ttatgatgaa agtagttcat gggctccata
     6481 tgatatagga atacttacag ggaatataac aacagctgca caagcactag ggcttggaag
     6541 ttgtataata ggaatggtta gaggtttatt tacacctgtt gaacaagggg atattgaagg
     6601 tcttgtgagt gtattggata aagaagatgt aaaggaatca gagtcaataa agatgaagtt
     6661 tgatacaaat aaaaaataca gagaactact tgatatacca gaagggtatt ctgtaccatt
     6721 tggtattgct gtaggtattc cagatggaaa tttaccaaat gctagagagg tagtttacaa
     6781 agtttccaga gtttaggata aagtattgca agaaccaatc agttacttga agaattaaac
     6841 cagtaatata caaataaatt gatggaagag gaagctattt aaaaagtagt ttagattttt
     6901 cattgtaaaa taattaaata aagaataagg tgataagtat tttatttatt atcttattct
     6961 tttttatttt tgttttgtag aatgataaaa ataaggatta agtgtaataa aatagaaaac
     7021 aagtatgtta attttaagta tatgctttaa aatttatcca agttaaaaat ttaagatatc
     7081 ttttcagtat aatggaaaaa atgtcaaaat attgaaatag ggaaaaaata gtgatataat
     7141 aaaaaaaata gagaaaaaaa gtaaccctta gttgtaaaag ggttcttttt ttttataata
     7201 aaatagctat aaaaatatac atatcaaatc aaattaagaa gtatttcatt tctaagaaat
     7261 atcctaacat aaaaacaaaa aatgttcata aatttaacta ttaacataga taatttttag
     7321 aaaagtatct aaattttcaa taaatagtaa cttttttaca acaaatggaa actatgtacg
     7381 taataattaa aataaaaaat attattttat atttataaac attgacattc taattctgta
     7441 gtgtaatcgt attttttatt aattcttata aaatcaatga aaataaggat atccatttta
     7501 aagtacataa atttaaaaat ccagaaaaat atacttaaat tatgtaagca aaagaggggt
     7561 tgaatcggct gaaaatgtaa tactagtaaa tagtacatca atcctagata ccctgtaagc
     7621 aactctattt tcatgtacta aaaatgtacc tattttatta actgaaaaga ataaattaaa
     7681 tgtaagtaca taataagaga taaaaaatta aaagcaaaaa atatttatat aaataaatat
     7741 aaaaaatgat ataatcccta ttaaaaaatt tatagatgat gaagaaatat caaaatctta
     7801 tattatagga agaggcactt ctttgccaaa atatatagaa aagcaatacc aaatccaaag
     7861 agaataggct gagaaaaaat aaaacaaatt caagagtaat agaaaagctt tatagaataa
     7921 agaactgaga actctttata taataaaaaa tggactttta agagaaaatg atttaataaa
     7981 tgatttaaca tggtgtttta gcaggtgaaa ataaatcacc agtaatgatt gtagaaaaat
     8041 tactttatga atctcaaaaa aacttagtta agagtaagag atttgataag ataactaaag
     8101 ttggggaaat gtgtttgatg agataaaaaa attagtaaaa tagtataatc taaataagaa
     8161 tattttgttg attaaactac actaatagta atattgtagg gggaaaagat gagagaagaa
     8221 tcgaactctt taagtttaga ccatatagaa attttctttg aacttttatc tcaaagtaca
     8281 gaagattata tatttttttg ggatataaat aggaataaat ttaaaatttc ttcagcaatt
     8341 tttgatgaat ttaacttaag taaagagata gaatctgatt tagtcaattg ttggagtaaa
     8401 attgtttatc cggatgatgt tcaaatatgg aaagatgata tacaagaatt actacatgga
     8461 aaaaaaggtg agcacaattt agaatatagg ttgattaata aatatgaaga aatagtatgg
     8521 atttcttgta gaggtaaagt ttatgtgtcg gatgacccaa aaactatatt tctagtcggg
     8581 agaataaaga atattggtga aaaaaataaa tttgattcta tcacaggaac gtggaataga
     8641 gaacaatttg aacatagaat gaattatctt ataaaagaga aaatttataa aaatggtgct
     8701 atgtttatca tggatattga taattttaaa aatataaatg aaaaatatgg acattcttat
     8761 ggtgacaaag tattgcgtgc aattgctact gaagtattag aatatcttcc aaaagatgta
     8821 agattgtata ggttagatgg agatgaattt gcattttttt accctatgtg tactaaagaa
     8881 actatagaga aaatatatga aaagatacag atgtatacaa atactcagca tgaaattgaa
     8941 tcaaataagt actattgcac agtgactgca ggagttgcta tgtatccaga agatggagat
     9001 aattatttag atttatttaa acatgctgat attgcattgg atatagcaaa aattagtggt
     9061 aaaaatagaa ttaagttttt ttcacaagag ctctatgaaa ataaactaaa ggtaatttca
     9121 atgcaacaaa aactaagaga gtgcgttgaa aataatttta atgactttga gctgtttttt
     9181 caaccccagg taaatgctgt tacaaaagag gtaatagggg cagaggtact tttaagatgg
     9241 cattcgagta cttatggaga agtatctcct gttgaattta taccaatact agaacagagt
     9301 aatttaatta taccagttgg gaagtggatt ataaaagaag ctgtaaaaca gtgtaaagaa
     9361 tggcataaaa taaatccaga ctttaaaata tctgtaaatg tatcgtatat tcagttaaaa
     9421 gaagattttt ttagagattt tatagtagaa tgtttagtag agtatcaatt aagacctgaa
     9481 tttttaattt tggaattaac agagaattgt tggataccag atataaattt actaaatgat
     9541 aaatttatta gtttaaaggg tataggagta tatattgcaa tagatgactt tggtacaggg
     9601 tattcatctt taaattacct taaagaactg tctgttaata taataaaaat tgaaagaagt
     9661 tttgttaaga atattacata taatagttat gaatatactt ttcttgaata tattattaaa
     9721 ttagctcata ttataaattt aaaagtttgt gttgagggaa tagagtctta tgaagagtat
     9781 gatatagtaa aaagcttagg tgtagatatt attcagggtt ttttatttgg aagacctgta
     9841 agtgcctctg aattttataa atttcaacta tcaaagtgat gtacataaaa tgagatatat
     9901 agtatataaa aatagggcaa agtggaagtt ttgaagttta ttaaaaattt acactttacc
     9961 ctattttgtt atgtcttcta taaaggatta atatactaca ctaactatta tatactttca
    10021 ctccaaattt agagattatt tcactagctt tttggacatc ttccttagtg gcatcaggaa
    10081 catcttttag ttcataatcg atacctaaac tttcccattt gtgtattccc atagaatggt
    10141 atggtagcaa ttcaaatctt tctacatttt tcaatgtagc tacaaattga cctagttttt
    10201 ctaaattatc tatatcatca gtaatgccag gaactaaaac atgtcttatc catacaggaa
    10261 tatttcttga ttctaagtgt ctagcaagtt ttaatgcctt atccatacca acaccagtca
    10321 aatcaataga tttttcctca accatatgtt ttaaatcaag taaaaccaaa tcagtattat
    10381 ctaaaactgg gtctatagtt tctatatcta cgaatccaga tgtatccaag caagtgtgta
    10441 tatcattttc ttttgctttt ttaaataatt cacttaaaaa ttctggttga agagtggatt
    10501 caccaccaga ggcagttata cctccaccag aaaacttcat aaaagaagta tacttaagag
    10561 catccgttat tatttcatca actgtgtatt cttttcctga tttaatatcc catgtatccc
    10621 tgttatgaca gtatttgcat ctaagaggac acccctgaaa aaataatata tatcttattc
    10681 ctggtccatc tacagtacca aacgtttcta tagaatgtat ctttccctta atcatagagt
    10741 tcacctcaca atatatttta ataaccttat caatagttat aatatttgtc taaaaaaggc
    10801 catcttatac cagaaataat tcaaatataa gatagccttt tatttttatt ttttacacag
    10861 tagtaatctt agttaaagta taaaaattta aagactaagc cattttaccg tggaatgttc
    10921 tgtttataac atctaattgt tgctctttag ttaattttat gaagttaact gcatatccag
    10981 atactcttat agttaattgt ggatattctt ctggatgttc catagcatct tctaaagtag
    11041 ctctatcaaa tacatttaca tttaagtgat gagcattttg agagaagtat ccatccatca
    11101 ttgcagaaag attgtttatt ctttcagtca tatcttttcc taaagcagca ggtactatag
    11161 agaaagtatt agatatacca tcttgagcat gttcatatgg taatttagca actgaagata
    11221 atgaagctaa agcaccatta gtatctctac catgcattgg gttagcacca ggagcaaatg
    11281 gagcaccagc tcttctacca tctggtgtat taccagtttt cttaccataa acaacatttg
    11341 aagttattgt aagtatagat tgagttggat aagaatttct ataagtttta ttttttctta
    11401 ttttattcat aaagctttca actaactcta cagctatact atcaactcta tcatcattgt
    11461 ttccgtattt tggataatct ccttctattt caaaatcaac agctattcct tgttcatttc
    11521 taatagtttt aaccttagca tgttttatag cagataatga gtctgcacaa actgataacc
    11581 cagccatacc acaagccata gttctaaata tatctctatc atgtaatgcc atttctaatg
    11641 cttcataaga atatttatca tgcatataat gtattacatt taaagtattt acatataatg
    11701 ttgctaacca atcagtgaat ggttcaaatt tactcataac ttcatcataa tctaagtact
    11761 cagaagttat aggttcaaat cttggtccaa cttgaacacc agatttctca tctacaccac
    11821 catttatagc gtataataaa gttttagcta agttaactct agctccaaag aactgcattt
    11881 gtttaccaat tctcattgca gatacacaac aagctatacc atagtcatct ccccagtatg
    11941 ctctcattaa atcatcattt tcatactgta ctgaacttgt atctatagat actttagaac
    12001 agaaatcttt gaatccttga ggtaatttag ttgaccatag aactgttaag tttggctctg
    12061 gagaaggtcc taatgtatat agagtattta acattctaaa tgagttttta gtaactaatg
    12121 ttctaccatc tattcccata cctcctatac actcagttac ccaagttggg tctccagaga
    12181 ataggttatt gtaatcagga gttcttagga acttaaccat tcttaatttc ataacaaaat
    12241 ggtccatata ctcttgtaat tcttcttctg ttacgattcc agcttttaaa tctctttcaa
    12301 agtatatatc taagaaagta gatgttctac ctaaagacat tgcagcacca ttttggtctt
    12361 taacagcacc taaataacca aagtataacc attgtacagc ttcttttgag ttagttgctg
    12421 gttttgatat atcaaatcca taagattcag ccattttttt aagttcattt aaagctgata
    12481 tttgctcagt tatttcttct cttaatctga taacttcctc atccatgcaa gaaacttcta
    12541 atgatttctt ttgctcattt ttatcttcta ttaaagcatc aacaccgtat agagcaactc
    12601 ttctgtagtc accaattatt ctacctctac cataagcatc tggaagacct gttattatac
    12661 cagattttct agcagctctc atttctggag tataaacatc aaaaactcct tggttgtgtg
    12721 tttttctgta tttagtaaat atatctttta tttctgggtc taattcatat ccaaaagctt
    12781 cgcatgaatt ttcaaccatc tttataccac catatggcat gacagctctt tttaaagggg
    12841 catccgtttg taacccaact attgtttcta attctttatc taaatatcct ggagcatatg
    12901 cagctattcc agaaacagtt tttgtatcaa catctaaagt accaccattt tctctttctt
    12961 ttttaaatag gaccattgct tcgtcccata attttttagt attttcagtg gcaccagcta
    13021 agaaagagtc atttccctca tatggagaat agtttaattg tataaactct ctaacattta
    13081 tttctttagt ccatcttcct gttttaaatc cttgccatgc attcattaag tatttcctcc
    13141 ttagatttaa atgataaata atactattaa aattattttt tatatactta atttatttta
    13201 tatatttttt taaaaatata ttttctaata taataatatc atattattcg tatcctgtat
    13261 acataaaaaa taaaaaattg tcgctttaaa gtataaaaca gattatatgt taataatgtg
    13321 gtgaaattat acccatatat aaataaaata aaacaaataa atagctaatt aaggagaaaa
    13381 tattttttat tttttgttta ggataaataa attttattat ggaaaaaaga aaaagcatac
    13441 atcttcacaa attgaatatt tatttaatag ggggaggtga tagattgttt atcacagtgg
    13501 tattattttt agtaggtttc ctattaatta ctaaaggcgc agatatcttt ataaattgca
    13561 ctgtagagat aggtagaaaa acgaatatat cagaaattat acttggagct actatagtta
    13621 gttttgcaac aacattacct gaatttacag tgtctctact tgcttctatt gatggtcata
    13681 ctactatgag tttaggaaat gcagttggtt ctataatatg taatacagga ttagcattag
    13741 ggttggttgt ttttataagt ccatttaatg tagataaaaa aatgtttttt tctaagtctt
    13801 tactattaat agtatccgtt atagttttaa ttttattgag tttagatgga gttataacaa
    13861 gaggtgattc attattactt ataataatat taatctttta tatggttaat aattatagaa
    13921 gtgtcgttgg aaaatctgat actaaaaata gaaatattaa aaataacact attaaagaca
    13981 tatctactaa aaaagggaaa aattatagag gtttttccgt attagaaatt gtaaaaatac
    14041 tattactttt tgctacagga ttgataatga tgataatagg ttcacaaatc cttatagaga
    14101 gtggagtaat aatagctagt tttttaaata tacctcaggg tatagtaagt ttaactataa
    14161 tagcacttgg aacttctctg ccagagatag tttcgtcgat tactgctata agaaaaaatc
    14221 atcatgaaat atcagtagga aatattttgg gtgcaaacat attgaatata gtatcagtaa
    14281 tagctgtatc agcaatacca aataacatac caatactatc tcaaaataga caattggata
    14341 taccattcat gatattatta ttattaatag taataatacc aacacttaaa tctaatagac
    14401 ttagtagaat acaaggtata ttgatgttat tcacatattt tctgtacata tcaattttgt
    14461 atttcatgta tattatttaa ctatactgtt taaaatatta attgcagaat atcctaaaaa
    14521 agtatataat ggataggtta agattaattg ataacggaga ggaataatat atgaatataa
    14581 caaagtttga agatttacca ataagtgagg gaataaaaaa agctatagct gaaatggggt
    14641 ttgaagaacc atctcctata caagcacagt ctatacctgc gattttgtca ggtaaagacg
    14701 ttataggaca agctcaaact ggtacgggaa aaacagctgc ttttagtatc cctatactag
    14761 aaacaataga cccaaataat agaagtttac aagctgtagt gctttgtcca acaagagaac
    14821 tggcaataca agtatcaaca gaaataagaa agttagctaa gtattcgcac ggaataaaga
    14881 cattacctat atatggtggc cagccaatag atagacaaat aaaatctcta aagagtggtg
    14941 tgcaagttgt aataggtaca ccaggacgta caatagacca tatcaatcgt aaaacattaa
    15001 agatggataa tgtaaaaatg attatattag atgaagcaga tgaaatgcta gatatgggat
    15061 ttagagaaga tatagaaatg attttaagta agattccaga agaaagacaa acaactttct
    15121 tttcagctac tatgccaaga ggaattttgg aattaactaa gagatatcaa aaagacccag
    15181 agcatataaa agttgttaga aaagaactta cagtttcaaa tacaaagcaa tactatatag
    15241 aaactaggtc atcaaataaa ttagaagtat tatgtagatt agtagatgta tatgacccta
    15301 aactatctgt tgttttctgt aatactaaga gaaaagcaga tgaattggta ggagatttac
    15361 aagcaagagg atattttgca gatgcattac atggtgactt aaaacagact caaagagata
    15421 tagtaatgga taagtttaga aatggtacaa tagatatact tgttgctact gatgttgcag
    15481 ctagaggtat agatgttgat gatgttgaat gtgtattcaa ctatgactta ccacaagatg
    15541 aagagtatta cgttcataga attggtagaa ctggtcgtgc tggtagagaa ggtatgtcat
    15601 ttacttttgt atttggaaaa gaaatgagaa agatgaaaga tatagaaaga tatactaaat
    15661 ctaaactaat aaagcataat ataccaacaa taactgatgt tgaagagaaa aaagtaggta
    15721 ctttctttgc acaagttaaa caaactatag aagaaggtca cttaactaaa caattacaat
    15781 ggttagaagg tttctgtaat gatgaagatt atgcaatggt tgatatagct gctgcacttg
    15841 taaaactatc tctaggtgaa gaaatgaaag aagaaataat agaagaaaaa cctagaagag
    15901 agcgtggaga tagaaaaggt ggtactggag ctaaagatgg tatgattaga ttatttatca
    15961 atattggtag aaatcaaaga gtacaagcaa aagatatagt tggtgctata gctggagaag
    16021 ttggaatacc aggtaaagta gtaggtacta tagatatata tgataagtat acttttgttg
    16081 aaatacctaa aaaagatgca aaaacagtaa ttgaaaagat gaaagatatt aagataaaag
    16141 gtaataagat taatatagaa aaagcaaata agaagaaaaa ataacgtata gtatacaaaa
    16201 ctataattag aaaatatata aaataatata ttggatattt aaaaagcctg atgtgttttc
    16261 aaaacagata gtagataatc tgtaagagtt aacagcataa ggcttttctt gttttatatg
    16321 atattatgat tttccagatc gttgtgaaaa atgtatatta gttccttttt ttcaaatctg
    16381 atttgaaaaa gagaatatta attatctata tagtttagat tataataaaa aatgtaagat
    16441 aacaaagggg agaaaaagat gtcaatgact tatatggacc ctagggtttt tttgatttta
    16501 gaaatggtgt tagaagaaga cacaaatact ctagaacaat tttcaaatgt tttgggtgtt
    16561 agtactagga ctatcagaaa ctatataaac cagattaatc gagaattaaa aggtggtatt
    16621 gctaaaatag ttaaaaatga aaaaggtaca tatagtataa gaattgagga tgaaagtaaa
    16681 ttaattgaga ttgtaaattt taatagagga aagctatcaa attttataaa tttaaattca
    16741 cctgatgaaa gaataaatta tgttcttgat attttaacta tgaccaacaa tccaattacc
    16801 atagatgatt tggctgaaga gatatgtata ggaagaacaa cattaataaa ggatttaaaa
    16861 aaagcagaca gaattttagc tgaatatgat ttagaattaa aaaggaagcc aaatataggt
    16921 atgattttag ttggtgaaga aatggatata agattattaa tccttgatag attgtatgaa
    16981 aattatattg atgtcttgga aaacgttaat cagataaatt tagttaaaga ttatgatatt
    17041 gaatgtctta gagatgaatt aaaaaagttg tttaaaaaag aagaactcta cataacagaa
    17101 caggtcttga gagatgttga aagatatata atcatgtctg tcttaagaaa tctaaatggg
    17161 tacaaatttg aaaagataga taagagattt gaagtgataa gatgttcaga tgaatatact
    17221 ttaggattaa agctcaaagc tttattggaa aagatattta atatagtttt aaatgaaaaa
    17281 gaaaccatat ttttgacaat gccattaata ggtagaaaag ctcctagtac aaaattagca
    17341 cttagcagta taaagataaa tgatagcgta aaggaagttg tagatgaagt aactagtttt
    17401 ttattagaga cctcagggat tgattttaaa gaagataaaa agcttataga aaatttggaa
    17461 tatcacttgt attttgcact taatagaatg agattcaaca taagggtaaa aaatcctctt
    17521 ttacaagaaa taaaggaaag atatacattt ccatatagtg ttgcaaagat agctgcaatg
    17581 ataattgagg aaaagtttga cttgaaaata tgtgaccacg aaataggata tatagcatta
    17641 cattttggaa gttattttga aaaaaattca agaaaagttc taaatgtaaa tagggtagct
    17701 gttgtttgtg gaacaggtct tgggactgca caacttatta gaataaagat aggaaaaata
    17761 cttggagaaa gtgtagaagt aaatactttt tctgatatag aagctaaagc taatttagat
    17821 ttattaaagt attatgattt agtatttaca acagtagatt tcaaaacaga cttagatgtt
    17881 ccaattttta gagtaaatgc gttatttaat gaagaaagta taaagaaaga gatagaaaat
    17941 gcaatacttt tgaaaaatag tataaatctt aagtctataa aaaagattga aaaaaatatc
    18001 ccttttatag gtaaattaat tgaagaggat aagttcttta tattgaataa gagtacgttt
    18061 atggaaaatt tagaagatat gttagaaaaa ttagttcata aaggaattat tgatatagag
    18121 ttcaaaaaaa aggtattaaa aagagaaaag aaatcttcaa cagcattaga taattatata
    18181 gcattgcctc actcagttaa tactaatggg gaaaatcttt atctgtcatt tggaatatta
    18241 gaaaaaccag tggtttggga tacaaaggaa attagaataa taatattgat gataattcca
    18301 gataagaatg tagatagtac agacctgata ataaaaagtt atgaagaagt acttgaagta
    18361 gggagagata aaaaaatgat agaagaactt gctaaagtaa aatcatttga tgaattttat
    18421 aaaataatta ccaagaggag gtaataatat ggacccagta atttttatta tagcatcact
    18481 tattacagtg tatttattaa atatttttct tggatactta cagcttaaag attttaataa
    18541 aaattatatt gaactaaaaa gaaaaggaag agtggcaata ggcagaaaaa aaggaagaat
    18601 ttcatcaggt acaattgtgt tgatacttat aaatgatgat ggtgtgattg tcgaaactag
    18661 aaaaatgcaa ggtgttacag tattggcaag agtaaagtca tttgaaggtt tggtaggaaa
    18721 gagcttagag tacatagaaa aatcagattt aactgtttac aataaactac ttaaaaaagc
    18781 aatactagat gcagtcaaac agtacataac atttgagaaa aataaatgtg aagatacaaa
    18841 taatctgagt actgatgaag agtgtaggat ggaagtagtt taaaaaaata tatttataag
    18901 gaggagttaa tatgacgcaa ttttttcaag tattagcaag tggtgcagat agttttatga
    18961 atttatttag ggcaggtggg caacaatttg taggttttgt aacagatata gtaccacttt
    19021 tagtagcatt attagttaca atgaatgcaa ttatcaactt tattggtaca gatagagtcg
    19081 agagacttgc aaaaaaatgt tctgcaaata tatttactag atatttgata ttaccagtcc
    19141 ttggtacatt tgtatttgca aacccaatga cactatcact aggaagattc ttgccagaaa
    19201 agtttaaacc tagttacttt gcagcagcaa gcttttcatg ccatacaatg aatggattat
    19261 tcccacatat caatccaggt gaactattta tatatctagg aattgcaaat ggtataacaa
    19321 ctttgggatt ctcaacagca gatttagcag ttagatattt gttagtgggt atagttgcaa
    19381 actttattaa aggtgtagtt actgactata caacaaaatt tgtagagaaa caacaaggaa
    19441 tagcattaaa ttcagatata aagattggat aattacttta aggtataaat aaatttttca
    19501 aataaaaaga tagtgtatag aggaggataa attatatggc atttaacaaa gttaaaatag
    19561 taaaaggttc tggaggatgg ggtggaccat tgataataga accaacagaa aagaagaata
    19621 aggtagtata tgtgactggg ggagcaaagc cagaaactgc agtaaaaata gctgaactta
    19681 caggatgtga gttaatagat ggattttcac agggtgttaa agatgatgaa atcgcatgtg
    19741 taataataaa ttgtggtgga acattgagat gtggtatata tcctcaaaag aaaataccga
    19801 ctataaatat aatgaaaaca ggaaaaagtg gaccattagc aatgtttata aaagaagata
    19861 tatatgtttc aggggtaaaa ccaacaaatg tagagcagtt ataattgagg tagtaggagg
    19921 gaagaaatat ggcttatgat actaaaaaga agataagtga gcagagtcaa ggaattgttg
    19981 caaaaatagg tatagcacta ggaaatgtga caagtactct ttttcaagct ggtagggata
    20041 ctatagatac attaatacat actatactgc cttttatggc tttcgttgca gtcctaatag
    20101 gtataataaa tcaatcaggt ataggaaatt gggtagccaa tttacttact ccattaatcg
    20161 gaaatgttgg aggtttagtt gtcttatcaa taatatgttc atttccgttg ttgtctccgt
    20221 ttcttggacc aggagctgtt atagctcaga tagtgggggt attggttggt acagaaatag
    20281 gaaaaggaaa tattccacca agtttagcat taccagcatt gtttgcaata aactcacaag
    20341 cagcttgtga ctttatacca gtaggtcttg gtcttgcaga agctgaacct gatacagttc
    20401 aagtaggagt tccgtcagtt ttatattcta gatttttaac aggtgcacca actgttctat
    20461 tagcatggtt agcaagcttt gggttatata aatagaagaa tactttataa ataattagag
    20521 agaggttcag ttgatatgga attaatatac agtactcaaa taaataaaat aggagataat
    20581 gcaaatgaat ttttacaaca taatatgttt gtaactttca aagataatgc tcctcaagag
    20641 ttagaaaatt actgttatat acattctgaa aataatttag taaaagaaat acttacgaat
    20701 gatgtgttat caataaataa tgttgattac aaaattactt ctgttggtga gttagtaaac
    20761 caaaatcttt cagaattagg tcatataaca tttaagttta caggtgaaaa agatggagca
    20821 attggaggga ccttatatct tgaagaaaaa gaaatagcac ctattggcgt aggtactata
    20881 ctaaaaataa ttagaaaata aaatttaaaa cgaatctaat catattataa gtaaattata
    20941 gataaaagtc aatttaacta atcaagttaa cttttaatgt gataaaaaag gggaaatgaa
    21001 aatgagaaaa gtggctttag ttgttggagg aggaagaagt ttaggtgaat atttatctaa
    21061 acatttagct aaagtaggtt atgatgttgc tattgcagat ttaaatgaag acaacgcaaa
    21121 aaaagtagct gaagaaatag aaactgaatt tggttgtaaa tctgtaggat atggttgtaa
    21181 tgccatagat gaaagacagg ttataagcac tgttaaagct attgaaaatg attttgatag
    21241 aatagattta ttagtgtaca atgcaggagt ggctgtaagt aaaaaaataa ctgattttgg
    21301 atacaaagat tttaaattta ttgtagacgt aaatttaaat gggtactttc tatttgcaag
    21361 agaagtatct aaagtcatga tagcaaaagg tattgagggt agaatcatac agataaattc
    21421 aaaatcaggt agagtgggaa gcaagcataa ttcaggatat tcagcaggta aatttgctgg
    21481 agtaggactt actcaaagtt tagcgctaga tttggcagaa cataagataa ctgtaaatgc
    21541 actgatgctc ggtaatttac ttgattctga tatgtttgaa agtttaattc ctcaatatgc
    21601 agtaaaattg ggaatacctg aatcagaagt caagcaagtt tatatagata aagtaccttt
    21661 aaaaagaggg tgtagattta tagatgttgc aaatatgctt acattttatg cttcaaagga
    21721 agctgaatac tgtactggtc aatctataaa tattactggt ggacaagtta tgtaaggttt
    21781 ttatggagtg tctcaagatg aactctttag ttcatgaggc actttttttg tataaaatta
    21841 aatatatttt catcatttaa acaatgaaaa agaggtttat ctttattttg agatagcctc
    21901 atttttgtaa ttaagaaaca tagtgtttaa gtaaacatat aagattgtta gttttttaaa
    21961 agagaatttt ttaataatat tatatttatg aagtattttt aaacttaatt tgattaaaaa
    22021 atagctacgc acacttgtga ctttagtcat gagttagtag ctaaattagt cagcatatag
    22081 ggaaacttat atgtagagat aggatagaac ctatccaata ctacttgaat tgctaaaaac
    22141 ccctaaagct aaccaaacta aaacataagg gtgaaataaa cctaagtgtg aaagttacga
    22201 aagtagaaaa aattggttag atggtataag gttaaatcct aagtacttta aaatgggcaa
    22261 tcagcaggta aactccgaat aggagaaact tcaacgacta ttcctcttga gggaagtaca
    22321 ctacaagtta tcggtagtgg aagtgggtag accttaacgg ataatgccga aggataagat
    22381 atagtctgtg cttgcatgaa agtgtaagaa gttcataaga gaactgcata ggtagtagcg
    22441 aacttatgtg aacgacaacc tcaaaaatga taaaagttaa gacttacggt tcttatatat
    22501 gttatttaaa aataaatata aaaaaccttc aaaatacttg ttttaagtaa ttgcatattt
    22561 taatattatg ataggtgatg aaaaatggaa gttactcatg gtagaggata tgcatataat
    22621 atgtcaaata tagacatcca gtattatggg gagacatctt tggaatccta gttatttcgt
    22681 tgctactgta tcatgctact gtatcagaaa atacagagga gcagataaga aaatatatta
    22741 ataatcagaa aaaacaagta aagtgaggtg atagtagtgg aaaaagctta taagtttaga
    22801 atgtatccaa ataaaaaaca gcaagagtta attaacaaaa cttttggatg ttgtagattt
    22861 gtatataata agtatcttgc taaaagaata gaagtttata aaaatgctaa agaaactttt
    22921 acttataaac agtgtagttc tgatttgacc aatttaaaaa aagaattaaa ttggcttaaa
    22981 gaacctgaca aattttcact acaaaacgcc ttaaaagatt tagataatgc atataaaaaa
    23041 ttctttaaag aaaaagcagg atttcctaaa tttaaatcaa agaaaattaa tagattttca
    23101 tataaaacta acttcacaaa tggaaa
//
